Epidemic of wild-origin H1NX avian influenza viruses in Anhui,China  

作　　者：Ye Ge Qiu-Cheng Yao Xian-Fu Wang Zhi-Qiang Fan Guo-Hua Deng Hong-Liang Chai Hua-Lan Chen Yu-Ping Hua 

机构地区：[1]College of Wildlife Resources,Northeast Forestry University,Harbin 150040,Heilongjiang Province,China [2]State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,China [3]Natural Protection&Management Station of Forestry Department Centre of Anhui Province,Hefei,Anhui Province,China [4]School of Life Sciences,Anqing Normal University,Anqing,Anhui Province,China

出　　处：《Infectious Diseases of Poverty》2017年第1期881-890,共10页贫困所致传染病（英文）

基　　金：This research was funded by the Special Key of Thirteen-Five national key research and development program“Key technology,research and development on biosafety”.The project number is 2016YFC1201600.

摘　　要：Background:As the natural hosts of avian influenza viruses(AIVs),aquatic and migratory birds provide a gene pool for genetic transfer among species and across species,forming transient“genome constellations.”This work describes the phylogenetic dynamics of H1NX based on the complete molecular characterization of eight genes of viruses that were collected from 2014 to 2015 in Anhui Province,China.Methods:Hemagglutination and hemagglutination inhibition tests were used to determine the hemagglutination(HA)activity of the HA subtypes.The entire genomes of the viruses were sequenced on an ABI PRISM 3500xl DNA Analyzer.The sequences were genetically analysed to study their genetic evolution using DNASTAR and MEGA 6.The pathogenic effects of the viruses were evaluated using mouse infection models.Results:Seven strains of the H1 subtype avian influenza virus were isolated.Phylogenetic analysis indicated natural recombination of the H1 influenza viruses between the Eurasian lineage and the North American lineage.Some genes had high sequence identity with A/bean goose/Korea/220/2011(H9N2),which is a typical case involving viral reassortment between the Eurasian lineage and the North American lineage.The results of infection experiments in mice showed that the viruses could acquire the ability to multiply in mouse respiratory organs without adaptation.Conclusions:These findings suggest that continued surveillance of wild birds,particularly migratory birds,is important to provide early warning of possible H1 influenza epidemics and to understand the ecology of the virus.

关 键 词：H1N1 ANSERIFORMES EPIDEMIC PHYLOGENETIC PATHOGENIC 

分 类 号：F42[经济管理—产业经济]