Comparative evaluation of a rapid diagnostic test,an antibody ELISA,and a pLDH ELISA in detecting asymptomatic malaria parasitaemia in blood donors in Buea,Cameroon  

作　　者：Tebit Emmanuel Kwenti Longdoh Anna Njunda Beltine Tsamul Shey Dickson Nsagha Nguedia Jules-Clement Assob Kukwah Anthony Tufon Dilonga Henry Meriki Enow George Orock 

机构地区：[1]Department of Medical Laboratory Sciences,Faculty of Health Sciences,University of Buea,P.B.63,Buea,Southwest Region,Cameroon [2]Department of Public Health and Hygiene,Faculty of Health Sciences,University of Buea,P.B.63,Buea,Southwest Region,Cameroon [3]Blood Bank,Regional Hospital Buea,P.B.32,Buea,Southwest Region,Cameroon [4]Programme in Medicine,Faculty of Health Sciences,University of Buea,Buea,Southwest Region,Cameroon

出　　处：《Infectious Diseases of Poverty》2017年第1期931-939,共9页贫困所致传染病（英文）

摘　　要：Background:In malaria endemic areas,infected blood donors serve as a source of infection to blood recipients,which may adversely affect their prognosis.This necessitates the proper screening of blood to be used for transfusion in these areas.The purpose of this study was to determine the prevalence of malaria parasitaemia in blood donors in Buea,Cameroon,and to evaluate the performance of a rapid diagnostic test(RDT),a malaria antibody enzyme-linked immunosorbent assay(ELISA),and a Plasmodium lactate dehydrogenase(pLDH)ELISA in the detection of asymptomatic malaria parasitaemia in the target population.Methods:In a prospective study conducted between September 2015 and June 2016,1240 potential blood donors were enrolled.The donors were screened for malaria parasites using Giemsa microscopy(GM)and a RDT.A sub-sample of 184 samples,comprising 88 positive and 96 negative samples,were selected for the evaluation of the pLDH ELISA and the antibody ELISA.The chi-square test and correlation analysis were performed as part of the statistical analyses.The statistical significance cut-off was set at P<0.05.Results:The prevalence of malaria parasitaemia in this study was found to be 8.1%(95%CI:6.6-9.7).The prevalence was not observed to be dependent on the age or sex of the participants.The RDT had a sensitivity(88.0%),specificity(99.1%),and negative predictive value(99.0%)higher than the ELISAs.The performance of the pLDH ELISA,which demonstrated the highest positive predictive value(91.6%),was generally comparable to the RDT.The sensitivity was lowest with the antibody ELISA(69.9%),which also demonstrated the highest false positive and false negative rates.The detection threshold for the pLDH(three parasites/μl)was lower compared to the RDT(50-60 parasites/μl).Non-significant positive correlations were observed between the parasite density and the pLDH titers and malaria antibody titers.Conclusions:Overall,the RDT and the pLDH ELISA demonstrated a perfectly correlated agreement with GM,meanwhile the antibody ELISA demon

关 键 词：Blood transfusion MALARIA RDT pLDH ELISA Malaria antibody ELISA Comparative evaluation Sensitivity Specificity Buea Cameroon 

分 类 号：R53[医药卫生—内科学]