Comparison of novel and standard diagnostic tools for the detection of Schistosoma mekongi infection in Lao People’s Democratic Republic and Cambodia  

作　　者：Youthanavanh Vonghachack Somphou Sayasone Virak Khieu Robert Bergquist Govert Jvan Dam Pytsje THoekstra Paul L.A.M.Corstjens Beatrice Nickel Hanspeter Marti Jürg Utzinger Sinuon Muth Peter Odermatt 

机构地区：[1]Faculty of Basic Sciences,University of Health Sciences,Ministry of Health,Vientiane,Lao People’s Democratic Republic [2]Swiss Tropical and Public Health Institute,P.O.Box,CH-4002 Basel,Switzerland [3]University of Basel,P.O.Box,CH-4003 Basel,Switzerland [4]National Institute of Public Health,Ministry of Health,Vientiane,Lao People’s Democratic Republic [5]National Centre for Parasitology,Entomology and Malaria Control,Ministry of Health,Phnom Penh,Cambodia [6]Ingerod,Brastad,Sweden [7]Department of Parasitology,Leiden University Medical Center,Leiden,The Netherlands [8]Department of Molecular Cell Biology,Leiden University Medical Center,Leiden,The Netherlands

出　　处：《Infectious Diseases of Poverty》2017年第1期1123-1135,共13页贫困所致传染病（英文）

基　　金：We are grateful to financial support of the Task Force for Global Health,Neglected Tropical Diseases Support Centre,the Department of Parasitology,Leiden University Medical Center and the Swiss Tropical and Public Health Institute.

摘　　要：Background:Given the restricted distribution of Schistosoma mekongi in one province in Lao People’s Democratic Republic(Lao PDR)and two provinces in Cambodia,together with progress of the national control programmes aimed at reducing morbidity and infection prevalence,the elimination of schistosomiasis mekongi seems feasible.However,sensitive diagnostic tools will be required to determine whether elimination has been achieved.We compared several standard and novel diagnostic tools in S.mekongi-endemic areas.Methods:The prevalence and infection intensity of S.mekongi were evaluated in 377 study participants from four villages in the endemic areas in Lao PDR and Cambodia using Kato-Katz stool examination,antibody detection based on an enzyme-linked immunosorbent assay(ELISA)and schistosome circulating antigen detection by lateral-flow tests.Two highly sensitive test systems for the detection of cathodic and anodic circulating antigens(CCA,CAA)in urine and serum were utilized.Results:Stool microscopy revealed an overall prevalence of S.mekongi of 6.4%(one case in Cambodia and 23 cases in Lao PDR),while that of Opisthorchis viverrini,hookworm,Trichuris trichiura,Ascaris lumbricoides and Taenia spp.were 50.4%,28.1%,3.5%,0.3%and 1.9%,respectively.In the urine samples,the tests for CCA and CAA detected S.mekongi infections in 21.0%and 38.7%of the study participants,respectively.In the serum samples,the CAA assay revealed a prevalence of 32.4%,while a combination of the CAA assay in serum and in urine revealed a prevalence of 43.2%.There was a difference between the two study locations with a higher prevalence reached in the samples from Lao PDR.Conclusions:The CCA,CAA and ELISA results showed substantially higher prevalence estimates for S.mekongi compared to Kato-Katz thick smears.Active schistosomiasis mekongi in Lao PDR and Cambodia might thus have been considerably underestimated previously.Hence,sustained control efforts are still needed to break transmission of S.mekongi.The pivotal role of highly sensitive diag

关 键 词：Cambodia Food-borne trematodes Kato-Katz Lao People’s Democratic Republic Point-of-care circulating cathodic antigen Schistosoma mekongi SEROLOGY Soil-transmitted helminths Up-converting phosphorlateral-flow circulating anodic antigen 

分 类 号：F42[经济管理—产业经济]