Identification of tick-borne pathogen diversity by metagenomic analysis in Haemaphysalis longicornis from Xinyang,China  

作　　者：Lu Zhuang Juan Du Xiao-Ming Cui Hao Li Fang Tang Pan-He Zhang Jian-Gong Hu Yi-Gang Tong Zhi-Chun Feng Wei Liu 

机构地区：[1]State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,20 Dong-Da Street,Fengtai District,Beijing 100071,People’s Republic of China [2]Affiliated Bayi Children’s Hospital,PLA Army General Hospital,5 Nan-Men-Cang,Dongcheng District,Beijing 100700,People’s Republic of China [3]National Engineering Laboratory for Birth Defects Prevention and Control of Key Technology,5 Nan-Men-Cang,Dongcheng District,Beijing 100700,People’s Republic of China [4]Center for Diseases Control and Prevention of Chinese Peoples’Armed Police Forces,Beijing 102613,China

出　　处：《Infectious Diseases of Poverty》2018年第1期440-447,共8页贫困所致传染病（英文）

基　　金：This study was funded by the Natural Science Foundation of China(81621005,81472005,81473023).

摘　　要：Background:A wide variety of pathogens could be maintained and transmitted by Haemaphysalis longicornis.The aim of this study is to systematically examine the variety of pathogens carried by Haemaphysalis longicornis,an importnatn vector,in tick-borne diseases epidemic area,and to estimate the risk of human infection imposed by tick bites.Methods:Adult questing ticks were collected in Xinyang,central China.Genomic DNA and RNA were extracted from 144 H.longicornis ticks individually,and sequenced respectively as the templates for high-throughput sequencing.Clean reads were compared against the database of NCBI nucleotide collection and specific PCR was performed to confirm the presence of pathogen.Phylogenetic analysis was performed to explore the evolutionary status of pathogens.Results:The assignment of reads to taxa based on BLASTN results revealed the existence of several potential pathogens,including Anaplasma spp.,Rickettsia spp.,Babesia sp.,as well as severe fever with thrombocytopenia syndrome bunyavirus(SFTSV).Comfirmantory PCR assays revealed the existence of Anaplasma bovis(13/144,9.03%),Anaplasma centrale(2/144,1.39%),Rickettsia heilongjiangensis(3/144,2.08%),Rickettsia sp.LON-13(1/144,0.69%),Rickettsia raoultii(5/144,3.47%),Babesia sp.(1/144,0.69%).SFTSV accounted for the highest detected pathogen with a positive rate of 18.75%(27/144).Three of the ticks(2.08%)were co-infected with SFTSV and A.bovis.Conclusion:Our study provided a broadened list of microorganism that harbored by H.longicornis.In previously unrecognized endemic regions,prokaryotic and eukaryotic infection including Anaplasma spp.,Rickettsiae spp.,and Babesia spp.should be considered,along with the well-known SFTSV for patients with tick bites history.A novel Babesia species was identified in local natural foci,which needs further investigation in the future.

关 键 词：Haemaphysalis longicornis PATHOGENS High-throughput sequencing 

分 类 号：O17[理学—数学]