Morphological and molecular characterization of invasive Biomphalaria straminea in southern China  

作　　者：Mohamed RHabib Shan Lv Yun-Hai Guo Wen-Biao Gu Claire JStandley Roberta LCaldeira Xiao-Nong Zhou 

机构地区：[1]Medical Malacology Laboratory,Theodor Bilharz Research Institute,Giza 12411,Egypt [2]National Institute of Parasitic Diseases,Chinese Center for Diseases Control and Prevention,Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China [3]Chinese Center for Tropical Diseases Research,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Shanghai 200025,China [4]Milken Institute,School of Public Health,George Washington University,Washington,D.C 20052,USA [5]Grupo de Pesquisas em Helmintologia e Malacologia Medica,Instituto Rene Rachou/Fiocruz,Av.Augusto de Lima,Belo Horizonte,MG 1715,Brazil

出　　处：《Infectious Diseases of Poverty》2018年第1期1245-1258,共14页贫困所致传染病（英文）

基　　金：This work was supported by the National Key Research and Development Program of China(No.2016YFC1202001);Research Fund for the Technical Reserves,National Institute of Parasitic Diseases,Chinese CDC(No.CB-1603);Mohamed R.Habib was supported by a Post-doctoral Programme from the Ministry of Science and Technology of China and National Center for International Research on Tropical Diseases(Shanghai,China);Roberta L.Caldeira is supported by a fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnologico/CNPq(No.304121/2014–2).

摘　　要：Background:Schistosomiasis is a common parasitic disease designated as a neglected tropical disease by the World Health Organization.Schistosomiasis mansoni is a form of the disease that is caused by the digenean trematode Schistosoma mansoni,transmitted through Biomphalaria spp.as an intermediate host.Biomphalaria was introduced to Hong Kong,China in aquatic plants shipments coming from Brazil and the snail rapidly established its habitats in southern China.Earlier studies of Biomphalaria spp.introduced to southern China identified the snails as Biomphalaria straminea,one of the susceptible species implicated in S.mansoni transmission in South America.However,recent molecular investigations also indicated the presence of another South American species,B.kuhniana,which is refractory to infection.As such,it is important to identify accurately the species currently distributed in southern China,especially with emerging reports of active S.mansoni infections in Chinese workers returning from Africa.Methods:We combined morphological and molecular taxonomy tools to precisely identify Biomphalaria spp.distributed in Guangdong Province,southern China.In order to clearly understand the molecular profile of the species,we constructed a phylogeny using mtDNA data(COI and 16S rRNA sequences)from six populations of Biomphalaria spp.from Shenzhen City in Guangdong Province.In addition,we examined the external morphology of the shell and internal anatomy of the reproductive organs.Results:Both morphological and molecular evidences indicated a close affinity between Biomphalaria spp.populations from Guangdong and B.straminea from Brazil.The shell morphology was roughly identical in all the populations collected with rounded whorls on one side and subangulated on the other,a smooth periphery,an egg-shaped aperture bowed to one side,and a deep umbilicus.The shape and number of prostate diverticula(ranged from 11.67 to 17.67)in Guangdong populations supports its close affinity to B.straminea rather than B.kuhniana.Molecular analys

关 键 词：Biomphalaria straminea Molecular taxonomy Schistosoma mansoni Intestinal schistosomiasis Susceptibility Invasive species 

分 类 号：F42[经济管理—产业经济]