手性流动相添加剂法拆分安妥沙星对映体  

Separation of antofloxacin enantiomers by HPLC using chiral mobile phase additive

作　　者：陈希 鄢雷娜 刘绪平 肖钦钦 段和祥 张文婷 陈伟康 CHEN Xi;YAN Leina;LIU Xuping;XIAO Qinqin;DUAN Hexiang;ZHANG Wenting;CHEN Weikang(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang 330029)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,南昌330029

出　　处：《分析试验室》2021年第8期918-921,共4页Chinese Journal of Analysis Laboratory

基　　金：江西省药品监督管理局科研项目(2019JS15)资助。

摘　　要：采用L-苯丙氨酸和CuSO_(4)作为手性流动相添加剂,建立液相色谱法拆分安妥沙星对映体的方法。考察了手性添加剂的种类、浓度及流动相pH等对安妥沙星对映体分离的影响。采用Welch Ultimate XBC_(18)色谱柱(4.6 mm×250 mm,5μm),优化流动相为8 mmol/L L-苯丙氨酸溶液(含4 mmol/L的CuSO_(4),pH 3.5)-甲醇(80∶20,V∶V),流速:1.0 mL/min,检测波长:302 nm,结果左、右旋安妥沙星峰达到基线分离,分离度为6.6。A method of HPLC was established for separating enantiomers of antofloxacin by using Lphenylalanine and copper sulfate as chiral mobile phase additives.The factors for chiral separation of antofloxacin,including the type of chiral additive,concentration of chiral additive and pH of mobile phase,were investigated.A Welch Ultimate XB-C_(18)column(4.6 mm×250 mm,5μm)was used,and the optimized mobile phase was 8 mmol/L L-phenylalanine solution(containing 4 mmol/L copper sulfate,pH 3.5)-methanol(80∶20,V∶V),flow rate was 1.0 m L/min and detection wavelength was 302 nm.The enantiomers of antofloxacin were completely separated with a resolution of 6.6.

关 键 词：手性流动相添加剂法 安妥沙星 对映体 拆分 

分 类 号：R917[医药卫生—药物分析学]