磺化聚苯乙烯纳米纤维作为金刚烷胺固相萃取介质的研究  

Sulfonated polystyrene nanofibers for solid phase extraction of amantadine

作　　者：王凤丽 胡奇杰 王东旭 谷贵章 陈柯 王艳艳 WANG Fengli;HU Qijie;WANG Dongxu;GU Guizhang;Chen Ke;WANG Yanyan(Huzhou Institute for Food and Drug Control,Huzhou 313000)

机构地区：[1]湖州市食品药品检验研究院,湖州313000

出　　处：《分析试验室》2021年第8期937-941,共5页Chinese Journal of Analysis Laboratory

基　　金：浙江省公益计划研究项目(LGC19B050001);湖州市公益性技术应用研究重点项目(2020GZ19)资助。

摘　　要：以电纺聚苯乙烯纳米纤维(PS NFs)为基底,通过磺化反应制得具有亲水性的磺化聚苯乙烯纳米纤维(SPS NFs),并通过静态和动态吸附实验考察其对金刚烷胺的吸附性能。据此建立了基于SPS NFs的固相萃取(SPE)法,并应用于动物源食品中痕量金刚烷胺的快速检测。结果表明,pH 4时,SPS NFs对金刚烷胺达到最优吸附,吸附动力学符合准二级动力学模型;优化SPS NFs的SPE条件(样液流速、洗脱条件等),结合超高效液相色谱-串联质谱(UPLC-MS/MS)检测鸡肉、鸡蛋、牛奶等样品中的金刚烷胺,2.0~20.0μg/kg加标水平的加标回收率为71.6%~87.5%,检测限为0.2μg/kg。与传统固相萃取法相比,本方法集分离、净化、浓缩功能于一体。Sulfonated polystyrene nanofibers( SPS NFs) was fabricated by electrospinning and sulfonation process,and its adsorption performance for amantadine was studied by using static and dynamic adsorption method. A method based on SPS NFs solid phase extraction( SPE) was established for rapidly determination of amantadine in animal food. The results showed that the maximum static adsorption capacity of SPS nanofibers for amantadine was 13. 7 mg/g at pH 4. The kinetic process of adsorption was found to follow the pseudo-secondorder kinetic model. The influence factors of SPS NFs-SPE were studied,such as sample flow rate,eluent rate and volume. Under the optimum conditions,the method of SPE coupled with UPLC-MS/MS was applied to the analysis of amantadine in chicken,egg and milk samples. The recoveries of spiked milk,chicken,and egg samples at the concentrations of 2. 0-20. 0 μg/kg were 71. 6%-87. 5%,and the limit of detection of the method was 0. 2 μg/kg. This method integrated separation, purification and concentration compared with traditional SPE methods.

关 键 词：纳米纤维 磺化聚苯乙烯 金刚烷胺 固相萃取 

分 类 号：O658.2[理学—分析化学] S859.84[理学—化学]