NB-UVB对黑素细胞黑素合成相关基因表达的影响  

Effect of NB-UVB on expression of genes related to melanin synthesis in melanocytes

作　　者：孙晶莹[1] 封青[1] 李研[1] 霍雪萍[1] 孙丽君[1] SUN Jingying;FENG Qing;LI Yan;HUO Xueping;SUN Lijun(Department of Dermatology,Central Laboratory of Shaanxi Provincial People's Hospital,Xi'an 710068,China)

机构地区：[1]陕西省人民医院中心实验室,陕西西安710068

出　　处：《中国麻风皮肤病杂志》2021年第10期625-628,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金(编号:81903218)。

摘　　要：目的:研究NB-UVB照射对黑素细胞的细胞活性、黑素合成、黑素合成相关基因表达的影响。方法:培养黑素细胞系PIG1,利用MTT法检测不同剂量NB-UVB(400、800、1200、1600、2000 mJ/cm 2)照射后细胞的增殖率,采用NaOH裂解法测定细胞中的黑素含量,RT-PCR和Western blot方法检测UVB照射后12个黑素合成相关基因Tyr、Tyrp1、Tyrp2、OA1、MART-1、Pmel17、VAT-1、OCSP、syntenin、CHCHD3、flotillin-1、GPNMB的mRNA和蛋白的表达。结果:NB-UVB照射剂量在400、800、1200 mJ/cm 2时,细胞活力无明显变化,照射剂量为1600、2000 mJ/cm^(2)时细胞活力明显下降。NB-UVB照射剂量为400、800、1200 mJ/cm^(2)时,随着照射剂量的增加,黑素的合成增加;NB-UVB照射后,12种黑素体蛋白中,Tyr、Tyrp2、GPNMB、Syntenin、MART-1的基因mRNA及蛋白表达显著升高。结论:UVB照射显著增加了黑素合成和相关基因的表达。Objective:To study the effects of NB-UVB irradiation on the melanocytes viability,melanin synthesis,and melanin synthesis-related gene expression.Methods:The melanocyte cell line PIG1 was cultured.The cell proliferation after different doses of UVB irradiation(400,800,1200,1600,2000 mJ/cm 2)was detected by MTT method.The melanin content was detected by NaOH lysis method.The expression of melanin synthesis-related genes,Tyr,Tyrp1,Tyrp2,OA1,MART-1,Pmel17,VAT-1,OCSP,syntenin,CHCHD3,flotillin-1,GPNMB after UVB irradiation were detected by RT-PCR and Western blot.Results:The cell viability decreased significantly after irradiated by NB-UVB radiation at dose of 1600 and 2000 mJ/cm 2.There was no significant change of cell viability after irradiated by NB-UVB radiation at dose of 400,800 and 1200 mJ/cm 2,and melanin synthesis increased with increasing radiation dose.After ultraviolet irradiation,of the 12 melanin synthesis-related genes,5 of Tyr,Tyrp2,GPNMB,Syntenin,MART-1 mRNA and protein were significantly increased.Conclusion:NB-UVB irradiation significantly increased the melanin synthesis and the expression of related genes.

关 键 词：窄谱中波紫外线 黑素细胞 黑素合成相关基因 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]