Detection of reassortant avian influenza A (H11N9) virus in environmental samples from live poultry markets in China  

作　　者：Ye Zhang Shu-Mei Zou Xiao-Dan Li Li-Bo Dong Hong Bo Rong-Bao Gao Da-Yan Wang Yue-Long Shu 

机构地区：[1]National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [2]Key Laboratory for Medical Virology,National Health and Family Planning Commission,Beijing 102206,China

出　　处：《Infectious Diseases of Poverty》2016年第1期499-504,共6页贫困所致传染病（英文）

基　　金：the National Basic Research Grant(973)Plan for financially supporting this research.

摘　　要：Background:Avian influenza viruses have caused human infection and posed the pandemic potential.Live poultry markets are considered as a source of human infection with avian influenza viruses.Avian influenza routine surveillance of live poultry markets is taken annually in China.We isolated the 2 H11N9 influenza virus from the surveillance program.To better understand the risk caused by these new viruses,we characterize the genetic and pathogenicity of the two viruses.Methods:Viral isolation was conducted with specific pathogen-free(SPF)embryonated chicken eggs.Whole genome was sequenced,and phylogenetic analysis was conducted.Results:Two H11N9 viruses were identified,with all 8 segments belonging to the Eurasian lineage.The HA,NA,M,NS and PA genes were similar to virus isolates from ducks,and the NP,PB2 and PB1 gene segments were most similar to those viruses from wild birds,indicating that the H11N9 viruses might represent reassortant viruses from poultry and wild birds.The HA receptor binding preference was avian-like,and the cleavage site sequence of HA showed low pathogenic.The NA gene showed 94.6%identity with the novel H7N9 virus that emerged in 2013.There was no drug resistance mutation in the M2 protein.The Asn30Asp and Thr215Ala substitutions in the M1 protein implied a potentially increased pathogenicity in mice.Both viruses were low-pathogenic strains,as assessed by the standards of intravenous pathogenicity index(IVPI)tests.Conclusion:Two reassortant H11N9 avian influenza viruses were detected.These viruses showed low pathogenicity to chickens in the IVPI test.Public health concern caused by the reassortant H11N9 viruses should be emphasized during the future surveillance.

关 键 词：Avian influenza H11N9 Genetic characterization REASSORTANT 

分 类 号：R37[医药卫生—病原生物学]