Impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest China  

作　　者：Shun-Xian Zhang Yong-Ming Zhou Wen Xu Li-Guang Tian Jia-Xu Chen Shao-Hong Chen Zhi-Sheng Dang Wen-Peng Gu Jian-Wen Yin Emmanuel Serrano Xiao-Nong Zhou 

机构地区：[1]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,People’s Republic of China [2]Key Laboratory for Parasite and Vector Biology,Ministry of Health of China,WHO Collaborating Center for Tropical Diseases,National Center for International Research on Tropical Diseases,Shanghai 200025,People’s Republic of China [3]Yunnan Provincial Center for Disease Control and Prevention,Kunming,People’s Republic of China [4]Centre for Environmental and Marine Studies,Departamento de Biología,Universidade de Aveiro,Aveiro,Portugal [5]Servei d’Ecopatologia de Fauna Salvatge,Departament de Medicina i Cirurgia Animals,Universitat Autònoma de Barcelona,Bellaterra,Spain

出　　处：《Infectious Diseases of Poverty》2016年第1期534-546,共13页贫困所致传染病（英文）

基　　金：Development of PCR to detect virus,Giardia lamblia and Cryptosporidium spp.were supported by a fund from the National Science and Technology Major Project(Grant number:2012ZX10004-220);The field epidemiological investigation and conducting bacterium culture were supported by a fund from the National Science and Technology Major Project(Grant number:2008ZX10004-011);The detection of B.hominis was supported by a fund from the National Natural Science Foundation of China(Grant number:81473022).

摘　　要：Background:Acute diarrhea is a global health problem,resulting in high morbidity and mortality in children.It has been suggested that enteric pathogen co-infections play an important role in gastroenteritis,but most research efforts have only focused on a small range of species belonging to a few pathogen groups.This study aimed to assess the impact of co-infections with a broad range of enteric pathogens on children aged below five years who suffer from acute diarrhea in southwest China.Method:A total of 1020 subjects(850 diarrhea cases and 170 healthy controls)were selected from four sentinel hospitals in Kunming,Yunnan province,southwest China,from June 2014 to July 2015.Stool specimens were collected to detect five virus(rotavirus group A,RVA;norovirus,NoV;Sapovirus,SaV;astrovirus,As;and adenovirus,Ad),seven bacterial(diarrheagenic Escherichia coli,DEC;non-typhoidal Salmonella,NTS;Shigella spp.;Vibrio cholera;Vibrio parahaemolyticus;Aeromonas spp.;and Plesiomonas spp.),and three protozoan(Cryptosporidium spp.,Giardia lamblia,and Blastocystis hominis,B.hominis)species using standard microbiologic and molecular methods.Data were analyzed using the partial least square regression technique and chi-square test.Results:At least one enteric pathogen was detected in 46.7%(n=397)of acute gastroenteritis cases and 13.5%(n=23)of healthy controls(χ^(2)=64.4,P<0.05).Single infection with RVA was associated with acute diarrhea(26.5%vs.5.8%,P<0.05).The prevalence of a single infection with B.hominis in diarrhea cases was higher than in healthy controls(3.1%vs.0.5%,OR=4.7,95%CI:1.01–112.0).Single infection with NoV GII was not associated with diarrhea(4.4%vs.3.5%,OR=1.2,95%CI:0.5–3.3).Single infections with bacterial species were not observed.The prevalence of co-infections with two enteric pathogens in diarrhea cases was higher than in asymptomatic children(20.1%vs.5.3%,P<0.05).RVA-NoV GII was the most common co-infection in symptomatic children(4.4%),with it aggravating the severity of diarrhea.Conclusions:Although i

关 键 词：CO-INFECTION DIARRHEA Bacteria Virus Intestinal protozoa CHILDREN Severity Kunming Yunnan China 

分 类 号：F42[经济管理—产业经济]