Prevalence of pathogenic free-living amoeba and other protozoa in natural and communal piped tap water from Queen Elizabeth protected area, Uganda  

作　　者：Celsus Sente Joseph Erume Irene Naigaga Julius Mulindwa Sylvester Ochwo Phillip Kimuda Magambo Benigna Gabriela Namara Charles Drago Kato George Sebyatika Kevin Muwonge Michael Ocaido 

机构地区：[1]Department of Wildlife and Aquatic Animal Resources(WAAR),School of Veterinary Medicine and Animal Resources(SVAR),College of Veterinary Medicine,Animal Resources and Biosecurity(COVAB),Makerere University,P.O.Box 7062,Kampala,Uganda [2]Department of Biomolecular Resources and Biolab Sciences,School of Bio-security,Biotechnical and Laboratory Sciences(SBLS),College of Veterinary Medicine,Animal Resources and Biosecurity(COVAB),Makerere University,P.O.Box 7062,Kampala,Uganda [3]Department of Biochemistry and Sports Science(BSS),College of Natural Sciences(CONAS),Makerere University,P.O.Box 7062,Kampala,Uganda [4]Medical Research Council(MRC)/Uganda Virus Research Institute(UVRI),Research Unit on AIDS,P.O.Box 49,Entebbe,Uganda [5]Department of Molecular Biology,Vrije Universiteit,Pleinlaan 21050,Brussels,Belgium

出　　处：《Infectious Diseases of Poverty》2016年第1期595-608,共14页贫困所致传染病（英文）

基　　金：The researchers would like to thank WaterNet,SIG-RISE and CARNEGIE-Next Generation of African Academics(NGAA-Carnegie),for supporting and funding this research,and Molecular Biology Laboratory,Makerere University for the equipment used in the diagnostics.

摘　　要：Background:Pathogenic water dwelling protozoa such as Acanthamoeba spp.,Hartmannella spp.,Naegleria spp.,Cryptosporidium spp.and Giardia spp.are often responsible for devastating illnesses especially in children and immunocompromised individuals,yet their presence and prevalence in certain environment in sub-Saharan Africa is still unknown to most researchers,public health officials and medical practitioners.The objective of this study was to establish the presence and prevalence of pathogenic free-living amoeba(FLA),Cryptosporidium and Giardia in Queen Elizabeth Protected Area(QEPA).Methods:Samples were collected from communal taps and natural water sites in QEPA.Physical water parameters were measured in situ.The samples were processed to detect the presence of FLA trophozoites by xenic cultivation,Cryptosporidium oocysts by Ziehl-Neelsen stain and Giardia cysts by Zinc Sulphate floatation technique.Parasites were observed microscopically,identified,counted and recorded.For FLA,genomic DNA was extracted for amplification and sequencing.Results:Both natural and tap water sources were contaminated with FLA,Cryptosporidium spp.and Giardia spp.All protozoan parasites were more abundant in the colder rainy season except for Harmannella spp.and Naegleria spp.which occurred more in the warmer months.The prevalence of all parasites was higher in tap water than in natural water samples.There was a strong negative correlation between the presence of Acanthamoeba spp.,Hartmannella spp.,Cryptosporidium spp.and Giardia spp.with Dissolved Oxygen(DO)(P<0.05).The presence of Cryptosporidium spp.showed a significant positive correlation(P<0.05)with conductivity,pH and Total Dissolved Solids(TDS);whereas the presence of Giardia spp.had only a strong positive correlation with TDS.Molecular genotyping of FLA produced 7 Acanthamoeba,5 Echinamoeba,2 Hartmannella,1 Bodomorpha,1 Nuclearia and 1 Cercomonas partial sequences.Conclusions:All water collection sites were found to be contaminated with pathogenic protozoa that could possibly

关 键 词：PREVALENCE FREE-LIVING PROTOZOA Water Queen Elizabeth Uganda 

分 类 号：Q95[生物学—动物学]