生物亲和色谱柱在天然产物筛选中的应用及发展  

Application and development of affinity chromatographic column in active components screening

作　　者：段俊 黄小玲 刘智鹏 陈灏文 林航[2] 郭嘉亮 DUAN Jun;HUANG Xiaoling;LIU Zhipeng;CHEN Haowen;LIN Hang;GUO Jialiang(School of Medicine,Foshan University,Foshan 528000,China;Pharmacy College,Jinan University,Guangzhou 510632,China)

机构地区：[1]佛山科学技术学院医学院,广东佛山528000 [2]暨南大学药学院,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2021年第4期367-378,共12页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81503031,81872832);广东省基础与应用基础研究基金自然科学基金项目(2019A1515010806);广东省教育厅重点领域专项(2020ZDZX2057);广东省教育厅教育科学“十三五”规划项目(2018GXJK201);佛山科学技术学院口腔医学科研培育项目。

摘　　要：天然产物拥有悠久的临床应用历史,是生物活性化合物的丰富来源.从天然产物中快速筛选获得活性成分仍然存在种种困难与挑战.生物亲和色谱柱是基于生物大分子与配基之间特异性作用,实现活性成分选择性分离的一种液相色谱分析方法,具有高特异性、快速、灵敏等优点,在天然产物的筛选中具有广阔应用前景.综述近年来生物亲和色谱柱在天然产物筛选中的应用发展,重点介绍生物亲和固定相和色谱载体.Natural products have a long history of clinical use,and are rich source of bioactive compounds.Rapid screening and identification of biologically active components from natural products remain a technical challenge.Bioaffinity chromatographic column is a kind of liquid chromatography method based on the specific interaction between biological macromolecule and its molecule ligand,to realize the selective separation of active components.Due to its advantages of high specificity,rapidity and sensitivity,bioaffinity chromatographic column has a broad application prospect in the natural products screening.In this review,the recent development of bioaffinity chromatographic column in natural products screening were summarized,with a particular emphasis on the reported bioaffinity stationary phases and chromatographic supports.

关 键 词：生物亲和色谱柱 天然产物 活性成分筛选 药物分析 

分 类 号：R917[医药卫生—药物分析学]