基于宏基因组学的新疆牧场羊潜在人兽共患病原菌调查  

Investigation of potential zoonotic pathogens in Xinjiang pasture sheep based on metagenomics

作　　者：陈紫昕 刘海灿[2] 屈勇刚[3] 邓云丽 宋德强 徐春生[3] 祁凤华[3] 万康林[2] 刘志广[2] 袁秀琴[1] CHEN Zixin;LIU Haican;QU Yonggang;DENG Yunli;SONG Deqiang;XU Chunsheng;QI Fenghua;WAN Kanglin;LIU Zhiguang;YUAN Xiuqin(School of Public Health,University of South China,Hengyang,Hunan 421001,China;State Key Laboratory of Infectious Diseases Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China;Animal Health Supervision Institute of the Eighth Division of Xinjiang Production and Construction Corps,Shihezi,Xinjiang 832000,China)

机构地区：[1]南华大学公共卫生学院,湖南省衡阳市421001 [2]中国疾病预防控制中心传染病预防控制所国家重点实验室,北京市102206 [3]石河子大学动物科技学院,新疆维吾尔自治区石河子市832003 [4]新疆生产建设兵团第八师石河子市动物卫生监督所,新疆维吾尔自治区石河子市832000

出　　处：《中南医学科学杂志》2021年第5期497-501,共5页Medical Science Journal of Central South China

基　　金：国家科技重大专项子课题(2018ZX10305409-003-004)。

摘　　要：目的探究新疆牧场羊携带微生物多样性及潜在人兽共患病原菌种类,为有效防治人兽共患病提供理论依据。方法选取新疆喀什和石河子市牧场羊群,同期采集羊口咽拭子和肛拭子样品,提取DNA,采用宏基因组二代测序,比对Minikrakenv2数据库,测序结果进行物种注释、多样性和差异性分析,了解两地区羊携带的细菌种类。结果共采集羊口咽拭子72份和肛拭子231份。门水平上,两样品占主导地位的微生物为变形菌门、拟杆菌门和厚壁菌门;属水平上,两样品的优势菌属均不相同,共检出10种主要以食源性传播为主的人兽共患病原菌:大肠弯曲杆菌、空肠弯曲杆菌、大肠杆菌、多杀性巴氏杆菌、鲍曼不动杆菌、沙门氏菌、铜绿假单胞菌、蜡样芽孢杆菌、化脓性链球菌和猪链球菌。经主坐标(PCoA)分析和相似性(ANOSIM)分析,两地区间羊携带细菌和潜在人兽共患病细菌的差异均有统计学意义(P<0.05)。结论新疆地区牧场羊口咽部和肛门部菌群存在多样性及地区差异性,检出的人兽共患病原菌以食源性传播为主,存在食品安全和疾病传播风险。Aim To investigate the bacterial and the species of potential bacterial zoonotic pathogens carried by sheep in large-scale pasture in Xinjiang,China,in order to provide a theoretical basis for effective prevention and control of zoonoses.Methods Both oropharyngeal swabs and anal swabs samples were collected at the same time from sheep of the pastures selected in the Kashgar and Shihezi regions of Xinjiang.The genomic DNA in the samples was extracted,macrogenomic sequencing was conducted with second-generation sequencing.The microbial species annotation,similarity and diversity analyses were performed by comparing the minikrakenv2 database to understand the species of bacteria carried by sheep in the two regions.Results 72 Oropharyngeal swabs and 231 anal swabs were collected from sheep.At the phylum level,the dominant microorganisms in the two samples were Proteobacteria,Bacteroides and Firmicutes;while at the genus level,the dominant genera were different in both samples.A total of 10 zoonotic pathogens that are mainly food-borne transmission were identified,including Campylobacter coli,Campylobacter jejuni,Escherichia coli,Pasteurella multocida,Acinetobacter baumannii,Salmonella,Pseudomonas guangdongensis,Bacillus cereus,Trueperella pyogenes and Streptococcus porcineus.The results of Principal Coordinates Analysis(PCoA)and Analysis of similarity(ANOSIM)analysis showed that there were statistically significant difference between bacteria carried by sheep in the two regions,and among the potential zoonotic bacterial species in the sheep(P<0.05).Conclusion The oropharyngeal and anal flora of sheep in Xinjiang pastures are diverse and regionally different,and zoonotic pathogens mainly transmitted by foodborne pathogens were detected,which presented a certain risk of food safety and disease transmission.

关 键 词：羊 人兽共患病 细菌多样性 宏基因组 

分 类 号：R195.4[医药卫生—卫生统计学]