人工湿地耦合微生物燃料电池强化高盐废水中的脱氮机理研究  

Study on the Mechanism of Constructed Wetland Coupled Microbial Fuel Cell to Enhance Denitrification in High-salt Wastewater

作　　者：路通 刘菲菲[1] 陈士强 刘光洲 LU Tong;LIU Fei-fei;CHEN Shi-qiang;LIU Guang-zhou(Institute of Marine Science and Technology,Shandong University,Qingdao 266237,China)

机构地区：[1]山东大学海洋研究院,山东青岛266237

出　　处：《环境科技》2021年第4期29-34,共6页Environmental Science and Technology

基　　金：国家科技重大专项(2017ZX07101003);山东省重大科技创新工程(2019JZZY010333)。

摘　　要：将人工湿地耦合微生物燃料电池(CW-MFC)体系用于高盐废水的处理,考察了其在高盐环境下的污染物去除效果,并通过高通量测序分析了CW-MFC体系与CW体系中微生物群落的差异,揭示了CW-MFC体系强化高盐废水中的脱氮机理。结果表明:将CW与MFC耦合形成的CW-MFC体系具有稳定的输出电压,并可持续地为阴极反硝化过程提供电子供体。在60 d的水质监测期间,2种盐度下CW-MFC体系出水中的硝态氮和亚硝态氮浓度均低于相应的CW体系。高通量测序表明CW-MFC体系阴极区和阳极区分别富集了较高丰度的反硝化菌和产电菌,且由MetagenomeSeq分析可知,阴极反硝化菌和阳极产电菌的富集是导致CW-MFC体系与CW体系在微生物群落组成上产生差异的主要原因。The constructed wetland coupled microbial fuel cell(CW-MFC)system was applied to the treatment of highsalt wastewater.The removal performance of CW-MFC system for contaminants in high-salt environment was investigated.Meanwhile,the difference of microbial community between CW-MFC system and CW system was analyzed through highthroughput sequencing method,and the mechanism of CW-MFC system to enhance denitrification in high-salt wastewater was revealed.The results showed that the CW-MFC system formed by embedding MFC into CW had a stable output voltage and can continuously provide electron donor for denitrification process in the cathode region.During the 60-days water quality monitoring period,the nitrate and nitrite concentrations of effluent in CW-MFC system were lower than those in CW system at two salinity levels.High-throughput sequencing showed that the CW-MFC system enriched higher abundance of denitrifying bacteria in the cathode and electricity production bacteria in the anode,respectively.According to the MetagenomeSeq analysis,the enrichment of denitrifying bacteria and electricity production bacteria was the main reason for the difference on microbial community composition between CW-MFC system and CW system.

关 键 词：人工湿地 微生物燃料电池 高盐废水 脱氮 微生物机理 

分 类 号：X5[环境科学与工程—环境工程]