中华跌打丸对斑马鱼骨折愈合作用研究  

Study on Effect of Zhonghua Dieda Pill on Fracture Healing of Zebrafish

作　　者：杜正彩[1] 俞航萍 陈章美 夏中尚 赵芳惠 梁云飞 徐懿乔 郝二伟 侯小涛 邓家刚 DU Zhengcai;YU Hangping;CHEN Zhangmei;XIA Zhongshang;ZHAO Fanghui;LIANG Yunfei;XU Yiqiao;HAO Erwei;HOU Xiaotao;DENG Jiagang(Guangxi University of Traditional Chinese Medicine,Guangxi Scientific Experimental Center of Traditional Chinese Medicine,Nanning,530200,China;Guangxi University of Traditional Chinese Medicine,Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning,530200,China;Hunter Biotechnology,Co.,Ltd,Hangzhou 310051,China;Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Guangxi Wuzhou Pharmaceutical(Group)Co.,Ltd,Wuzhou,543000,China)

机构地区：[1]广西中医药大学科学实验中心,南宁530200 [2]广西中医药大学广西中药药效研究重点实验室,南宁530200 [3]杭州环特生物科技股份有限公司,杭州310051 [4]成都中医药大学,成都611137 [5]广西梧州制药(集团)股份有限公司,广西梧州543000

出　　处：《中国现代应用药学》2021年第12期1454-1460,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：广西科技重大专项(桂科AA17202040-2);广西中医药大学校级重点硕士研究生科研创新项目(YCSZ20190022);广西中药药效研究重点实验室项目(19-050-39);广西农作物废弃物功能成分研究协同创新中心建设项目。

摘　　要：目的应用斑马鱼研究中华跌打丸促骨折愈合、抗炎、促血管形成及软骨损伤修复作用。方法采用顿性棱刀挤压斑马鱼尾鳍建立骨折模型,评价其骨折愈合及抗炎作用;用转基因血管荧光斑马鱼评价其促血管形成作用;地塞米松建立斑马鱼软骨损伤模型,评价其软骨损伤修复作用;通过Q-PCR检测中华跌打丸对TNF-α、IL-1β、BMP-2和BMP-7基因表达的影响。结果与模型对照组比较,中华跌打丸在浓度为每尾2 mg时,骨折愈合促进作用为20.8%(P<0.05);浓度在15.6,31.3和62.5 mg∙L^(-1)时炎症消退作用分别为44.6%,50.6%和52.6%(P<0.05),且伴随TNF-α和IL-1β基因的表达有不同程度下调(P<0.05或P<0.01);浓度为1000,2000 mg∙L^(-1)时,促血管形成作用分别为19.7%和49.9%(P<0.01或P<0.001);中华跌打丸通过上调BMP-2和BMP-7基因的表达起到软骨修复作用(P<0.01或P<0.001),浓度为500 mg∙L^(-1)时,软骨损伤修复作用为42.0%(P<0.05)。结论中华跌打丸对斑马鱼有促进骨折愈合、抗炎、促血管及软骨损伤修复作用,且通过下调TNF-α和IL-1β的表达起到消炎作用,上调BMP-2和BMP-7基因的表达起到软骨修复作用。OBJECTIVE To study the antifracture healing,anti-inflammatory,angiogenesis and repair of cartilage damage effects of Zhonghua Dieda pill by using zebrafish.METHODS The fracture model was established by squeezing the tail fin of zebrafish by a blunt edge knife,and the effect of Zhonghua Dieda pill on fracture healing and anti-inflammatory was then evaluated;transgenic vascular fluorescence zebrafish was used to evaluate the angiogenic effect;cartilage damage model was established by treating zebrafish with dexamethasone,and then the effect of cartilage damage reparing was evaluated;the effects of Zhonghua Dieda pill on the gene expressions of TNF-α,IL-1β,BMP-2 and BMP-7 were measured by Q-PCR.RESULTS Compared with the model control group,the fracture healing effect of the Zhonghua Dieda pill was 20.8%(P<0.05)when concentration was 2 mg per tail.With down-regulating the expression of TNF-αand IL-1βgenes(P<0.05 or P<0.01),the anti-inflammatory effect of Zhonghua Dieda pill was 44.6%,50.6%and 52.6%at the 15.6,31.3 and 62.5 mg∙L^(-1)concentration(P<0.05);the angiogenesis effect of Zhonghua Dieda pill was 19.7%and 49.9%at the 1000,2000 mg∙L^(-1)concentration groups(P<0.01 or P<0.001);Zhonghua Dieda pill showed cartilage damage repairing effect by up-regulating the expression of BMP-2 and BMP-7 genes and result in 42.0%effect of cartilage damage repairing on zebrafish model at 500 mg∙L^(-1)concentration(P<0.05).CONCLUSION Zhonghua Dieda pill can promote fracture healing,anti-inflammatory,angiogenesis and cartilage damage repairing in zebrafish,and play an anti-inflammatory role by down-regulating the expression of TNF-αand IL-1βand play a cartilage repairing by up-regulating the expression of BMP-2 and BMP-7 genes.

关 键 词：中华跌打丸 斑马鱼 骨折愈合 抗炎 促血管形成 软骨损伤修复 

分 类 号：R285.5[医药卫生—中药学]