HPLC-FLD法测定不同产地林蛙卵和林蛙油中雌二醇的含量  

Determination of estradiol in eggs and oil of Rana Dybowskii by HPLC-FLD

作　　者：刘芳馨[1] 王月洁 许佳明[1] 李季泽 赵寰宇 徐阳 鲍慧玮[1] LIU Fang-xin;WANG Yue-jie;XU Jia-ming;LI Ji-ze;ZHAO Huan-yu;XU Yang;BAO Hui-wei(College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117;Department of Pharmacy,Baicheng Medical College,Baicheng Jilin 137000)

机构地区：[1]长春中医药大学药学院,长春130117 [2]白城医学高等专科学校药学系,吉林白城137000

出　　处：《中南药学》2021年第8期1634-1636,共3页Central South Pharmacy

基　　金：2019年度长春中医药大学科学研究发展基金项目。

摘　　要：目的建立HPLC-FLD法测定林蛙卵和林蛙油中雌二醇的含量。方法采用Extend C_(18)(250 mm×4.6 mm,5 μm)色谱柱;流动相为甲醇-水(55∶45,V/V);流速为1.0 mL·min^(-1);柱温为40℃;荧光检测器激发波长为282 nm,发射波长为315 nm;进样量为20 μL。结果雌二醇质量浓度在0.22~21.68 μg·mL^(-1)内与峰面积呈良好的线性关系(r=0.9999);精密度、重复性和稳定性均良好(RSD<2.0%),平均加样回收率为100.4%(RSD为1.4%)。结论该方法操作简单、准确、易重复,可用于林蛙卵和林蛙油中雌二醇的含量测定。Objective To determine the content of estradiol in eggs and oil of Rana Dybowskii by HPLC-FLD.Methods The Extend C18(250 mm×4.6 mm,5μm)column was used with mobile phase consisting of methanol-water(55:45,V/V).The flow rate was 1.0 mL·min^(-1) and the column temperature was 40℃.The fluorescence detector excitation wavelength was 282 nm,and the emission wavelength was 315 nm.The injection volume was 20μL.Results There was a good linearity between the estradiol concentration and the peak area at 0.22-21.68μg·mL^(-1)(r=0.9999).The precision test,repeatability test and stability test were all good(RSD<2.0%),and the average recovery was 100.4%(RSD=1.4%).Conclusion This method is simple,accurate and repeatable,and can be used to determine the content of estradiol in eggs and oil of Rana Dybowskii.

关 键 词：林蛙卵 林蛙油 高效液相色谱-荧光检测法 雌二醇 

分 类 号：R927[医药卫生—药学]