肝细胞癌原位与皮下PDX模型的方法学比较分析研究  

Methodological analysis of orthotopic and subcutaneous PDX model of hepatocellular carcinoma

作　　者：朱瑞敏 李宝亮[2] 路亚岚 詹相文 刘益豪 韩云林 秦川 高苒 ZHU Ruimin;LI Baoliang;LU Yalan;ZHAN Xiangwen;LIU Yihao;HAN Yunlin;QIN Chuan;GAO Ran(Chinese Academy of Medical Science&Comparative Medicine Center,Peking Union College,National Human Diseases Animal Model Resource Center,NHC Key Laboratory of Human Disease Comparative Medicine,Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases,Institute of Medical Laboratory Animal Science,Beijing 100021;China.2.Beijing Ditan Hospital,Capital Medical University,Beijing 100015)

机构地区：[1]中国医学科科学院医学实验动物研究所,北京协和医学院比较医学中心,国家人类疾病动物模型资源库国家卫生健康委员会人类疾病比较医学重点实验室北京市人类重大疾病实验动物模型工程技术研究中心,北京100021 [2]首都医科大学附属北京地坛医院,北京100015

出　　处：《中国实验动物学报》2021年第4期512-518,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：中国医学科学院医学与健康科技创新工程经费资助(2016-I2M-3-019,2019-I2M-1-004,2019-I2M-1-003);教育部高等学校学科创新引智基地(B20095)。

摘　　要：目的构建肝细胞癌(hepatocellular carcinoma,HCC)原位与皮下人源肿瘤异种移植(patient-derived xenografts,PDX)模型,并比较分析两者生长和病理学特点。方法收集肝癌患者的新鲜肿瘤组织,接种肝和皮下组织,比较分析两者生长特性、病理特征、成瘤率和成瘤时间。结果本次研究成功构建肝癌原位和皮下PDX模型,两者与患者原发肿瘤病理特征一致,其中原位和皮下综合成瘤率分别为50%(3/6)和20%(3/15),平均成瘤时间为30 d和68 d。结论肝癌原位PDX模型成瘤率高,对于难生长肿瘤提供了一个新的建模方法,皮下成瘤操作和观察较为简单,在具体科研实践中应根据情况使用合适方法。它们为深入探索HCC的发病机制,及药物筛选提供有效的动物模型。Objective Establish an orthotopic and subcutaneous patient-derived xenografts(PDX)model of hepatocellular carcinoma(HCC)and compare their growth and pathological characteristics.Methods Fresh tumor tissues from HCC patients from Beijing Ditan Hospital were collected,and implanted in NCG immunodeficiency mice liver and subcutaneous tissues.Their cell growth,pathological characteristics,tumor formation rate,and tumor formation time were analyzed carefully.Results This study established orthotopic and subcutaneous PDX model successfully,and both of them were consistent with the primary tumor pathological characteristics.The tumor formation rate were 50%(3/6)and 20%(3/15)in orthotopic and subcutaneous tissues,respectively.The average tumor formation time were 30 and 68 days in the orthotopic PDX model.Conclusions The orthotopic model has a higher tumor formation rate,it provides a new choice for difficult tumor growth.The subcutaneous model were easy to operate and observe.Hence,the two models were choose according to the specific scientific research practice.They provides effective animal models for further exploration of the pathogenesis of HCC and drug screening.

关 键 词：肝细胞癌 人源肿瘤异种移植模型 原位移植 皮下移植 

分 类 号：Q95-33[生物学—动物学]