牛奶中β-内酰胺酶检测方法的研究  

Study on the Detection of β-lactamase in Milk

作　　者：李滨 张俊杰 徐洁 张磊 LI Bin;ZHANG Junjie;XU Jie;ZHANG Lei(College of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222000,China)

机构地区：[1]江苏海洋大学食品科学与工程学院,江苏连云港222000

出　　处：《现代食品》2021年第11期186-189,共4页Modern Food

基　　金：江苏省研究生科研与实践创新计划项目(SJCX19_1000)。

摘　　要：目的:研究一种快捷、简便、低成本、准确度高的检测牛奶中β-内酰胺酶残留的方法。方法:通过硫酸铜-紫外分光光度计法优化反应条件,降低最低检出限。结果:由硫酸铜-紫外分光光度计法优化结果显示可将牛奶中β-内酰胺酶的最低检出限降低至5 U·mL^(-1)。结论:硫酸铜-紫外分光光度计法有效降低了牛奶中β-内酰胺酶的最低检出限,与传统微生物法等方法相比,本研究开发的方法操作简单方便,成本低,准确度高,稳定性好,可满足目前市场需求。Objective:To study a fast,simple,low-cost,and highly accurate method for detecting β-lactamase residues in milk.Method:The reaction conditions were optimized by copper sulfate-ultraviolet spectrophotometer method to reduce the minimum detection limit.Results:The optimized results of the copper sulfate-ultraviolet spectrophotometer method showed that the minimum detection limit of β-lactamase in milk could be reduced to 5 U·mL^(-1).Conclusion:The copper sulfate-UV spectrophotometer method effectively reduces the minimum detection limit of β-lactamase in milk.Compared with traditional microbiological methods and other methods,the method in this study is simple and convenient to operate,low cost,and accurate high degree of stability,good stability,more suitable for the current market demand.

关 键 词：Β-内酰胺酶 检测方法 最低检出限 硫酸铜 紫外分光光度计 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]