Sirt1抑制由ox-LDL诱导的人冠状动脉内皮细胞的炎症反应  

Sirt1 INHIBIT THE INFLAMMATORY RESPONSE OF HUMAN CORONARY ARTERY ENDOTHELIAL CELLS INDUCED BY ox-LDL

作　　者：魏明慧 孙浩荣 王静文 薛明明[1] WEI Ming-hui;SUN Hao-rong;WANG Jing-wen(Inner Mongolia Medical University,Hohhot 010059 China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010059

出　　处：《内蒙古医科大学学报》2021年第2期113-117,139,共6页Journal of Inner Mongolia Medical University

基　　金：内蒙古自治区卫生计生科研计划基金项目(201701044);内蒙古自治区科技计划项目(2020GG0238)。

摘　　要：目的:探讨Sirt1对动脉粥样硬化中人冠状动脉内皮细胞炎症反应的影响。方法:选取人冠状动脉内皮细胞,分别用不同浓度的ox-LDL诱导并筛选出最适浓度建立体外动脉粥样硬化细胞模型;将细胞分为正常对照组、空质粒对照组、空质粒+ox-LDL组、Sirt1过表达+ox-LDL组、Sirt1干扰+ox-LDL组;用荧光显微镜观察质粒转染情况;qPCR和Western Blot验证Sirt1、ICAM-1的表达情况;用ELISA法检测炎症因子IL-1、hs-CRP的表达水平。结果:100 mg/L ox-LDL诱导体外动脉粥样硬化细胞模型效果最佳;与正常对照组和空质粒对照组相比,空质粒+ox-LDL组ICAM-1、IL-1、hs-CRP表达升高;与空质粒+ox-LDL组相比,Sirt1过表达+ox-LDL组ICAM-1、IL-1、hs-CRP表达降低,而Sirt1干扰+ox-LDL组则升高。结论:Sirt1能够抑制人冠状动脉内皮细胞中由ox-LDL诱发的炎症反应。Objective:Investigate the effect of Sirt1 on inflammatory response of human coronary artery endothelial cells in atherosclerosis.Methods:The human coronary artery endothelial cells were selected to induce and screen out the optimal concentration with different concentrations of ox-LDL to establish an in vitro atherosclerotic cell model.The cells were divided into normal control group,empty plasmid control group,empty plasmid+ox-LDL group,Sirt1 overexpression+ox-LDL group and Sirt1 interference+ox-LDL group by different treatment methods.The plasmid transfection was observed by fluorescence microscope,the expression of Sirt1、ICAM-1 was verified by qPCR and Western Blot,and the expression level of inflammatory factor IL-1、hs-CRP was detected by ELISA method.Results:100 mg/L ox-LDL can effectively induce atherosclerotic cell model in vitro;Compared with normal control group and empty plasmid control group,the ICAM 1、IL-1、hs-CRP expression of empty plasmid+ox-LDL group was increased;Compared with empty plasmid+ox-LDL group,the expression of Sirt1 overexpression group was decreased.Conclusion:Sirt1 can inhibit ox-LDL induced inflammatory response in human coronary artery endothelial cells.

关 键 词：SIRT1 人冠状动脉内皮细胞 炎症因子 

分 类 号：R331.33[医药卫生—人体生理学]