利用功能反应模型评价球孢白僵菌对东亚小花蝽捕食二斑叶螨的影响  

Evaluation of the effects of Beauveria bassiana on the predation of Tetranychus urticae(Acari:Tetranychidae)by Orius sauteri(Hemiptera:Anthocoridae)using functional response model

作　　者：陈亚丰 王甦[2] 邸宁 金道超[1] CHEN Ya-Feng;WANG Su;DI Ning;JIN Dao-Chao(Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agricultural and Rural Affairs, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China)

机构地区：[1]贵州大学昆虫研究所,贵州山地农业病虫害重点实验室,农业农村部贵阳作物有害生物科学观测实验站,贵阳550025 [2]北京市农林科学院植物保护环境保护研究所,北京100097

出　　处：《昆虫学报》2021年第8期967-975,共9页Acta Entomologica Sinica

基　　金：北京市农林科学院青年科学基金项目(QNJJ201917);北京市优秀人才培养资助项目(2018000020060G181);国家自然科学基金青年基金项目(1901945);北京市农林科学院创新能力建设专项(KJCX20200420);北京市科技计划项目(Z201100008020014)。

摘　　要：【目的】天敌昆虫和生防菌剂的应用都是害虫生物防治的有效手段,在生产中通过两者联合进行害虫的生物防治具有极强的应用前景和潜在的防控增效作用,然而生防菌对天敌的安全性评价是必不可少的。本研究旨在评估球孢白僵菌Beauveria bassiana与东亚小花蝽Orius sauteri联合应用进行害虫生物防治的可行性。【方法】测定了东亚小花蝽5龄若虫经2种不同浓度的球孢白僵菌孢悬液(常规浓度:1×10^(7)孢子/mL;低浓度:5×10^(3)孢子/mL)和孢子粉(1×10^(7)孢子/头)处理后成虫羽化率以及经上述处理后的东亚小花蝽5龄若虫对不同密度下的二斑叶螨Tetranychus urticae雌成螨的捕食能力。【结果】东亚小花蝽5龄若虫经白僵菌孢子粉(1×10^(7)孢子/头)处理后,成虫羽化率显著降低,其余处理的成虫羽化率与对照无显著差异。3个处理组中东亚小花蝽5龄若虫对二斑叶螨雌成螨的捕食功能反应均符合Holling II型,搜寻效应随猎物密度增加逐渐降低。经低浓度球孢白僵菌孢悬液(5×10^(3)孢子/mL)处理的东亚小花蝽5龄若虫的理论日最大捕食量(Na-max)最高,猎物处理时间最短(Th),搜寻效应回归线随猎物密度增加下降趋势最小;而球孢白僵菌孢子粉处理的东亚小花蝽5龄若虫理论日最大捕食量最低,猎物处理时间最长。【结论】低浓度的白僵菌孢悬液对东亚小花蝽5龄若虫对二斑叶螨雌成螨的捕食能力无影响,而孢子粉对该天敌5龄若虫捕食二斑叶螨雌成螨造成不利影响。本研究初步证明低浓度白僵菌孢悬液与东亚小花蝽联合应用防控二斑叶螨具有可行性。【Aim】The application of natural enemies and biocontrol bacteria are effective means of pest biological control,and the combined application of the two kinds of agents have strong application prospects and potential of synergistic effect on biological control in the production.However,it is essential to evaluate the safety of biocontrol bacteria on natural enemies.This study aims to assess the biocontrol potential of combined release of Beauveria bassiana and Orius sauteri for biological control of pests.【Methods】We measured the emergence rate of O.sauteri adults after the 5th instar nymphs were treated with two different concentrations of B.bassiana spore suspensions(routine concentration:1×10^(7) conidia/mL;low concentration:5×10^(3) conidia/mL)and spore powder(1×10^(7) conidia/individual)and the predation ability of the 5th instar nymphs of O.sauteri subjected to the above treatments to female adults of Tetranychus urticae under different prey densities.【Results】The emergence rate of O.sauteri adults was significantly reduced after the 5th instar nymphs were treated with B.bassiana spore powder(1×10^(7) conidia/individual),and there was no significant difference in adult emergence rate between the other treatments and control.The predation of the 5th instar nymphs of O.sauteri in the three treatment groups on female adults of T.urticae fitted with HollingⅡfunctional response models,and the searching efficiency reduced with the increasing of prey densities.Specifically,the 5th instar nymphs of O.sauteri treated with B.bassiana spore suspension at a low concentration(5×10^(3) conidia/mL)showed the highest daily maximal predation amount(Na-max)and the shortest handling time(Th),and the regression of searching efficiency showed the lowest decreasing trend with the increasing of prey density.However,the 5th instar nymphs of O.sauteri treated with B.bassiana spore powder had the lowest Na-max and the longest Th.【Conclusion】Low concentration of B.bassiana spore suspension does not affect the pre

关 键 词：东亚小花蝽 二斑叶螨 球孢白僵菌 生防菌 天敌昆虫 Holling模型 搜寻效应 生物防治 

分 类 号：Q968[生物学—昆虫学]