2型糖尿病db/db小鼠血管内皮细胞原代培养和生长曲线检测  

Primary Culture and Growth Curve Detection of Vascular Endothelial Cells in Type 2 Diabetic db/db mice

作　　者：陈雄林[1] 杨耀防[2] 熊含春[3] CHEN Xionglin;YANG Yaofang;XIONG Hanchun(Department of Medical Genetics and Histology&Embryology,School of Basic Medical Sciences of Jiujiang University,JiuJiang,Jiangxi 332000;Jiujiang University,JiuJiang,Jiangxi 332000;Department of Anesthesiology,the First Affiliated Hospital of Nanchang University,Nan Chang,Jiangxi 330006,China)

机构地区：[1]九江学院基础医学院医学遗传学与组织胚胎学教研室 [2]九江学院,江西九江332000 [3]南昌大学第一附属医院麻醉科,江西南昌330006

出　　处：《九江学院学报（自然科学版）》2021年第2期88-90,共3页Journal of Jiujiang University：Natural Science Edition

基　　金：国家自然科学基金项目(编号81960159);江西省教育厅科技研究项目(编号GJJ190004);江西省卫生健康委员会科技计划项目(编号201930009)的成果之一。

摘　　要：目的探讨糖尿病小鼠血管组织块血管内皮细胞原代培养方法,并检测其生长曲线。方法分离2型糖尿病db/db小鼠胸主动脉和腹主动脉,采用组织块贴壁法获得血管内皮细胞,通过传代对细胞进纯化,并对细胞进行生长曲线检测。结果血管组织块培养可快速高效获得的血管内皮细胞,并且细胞特性稳定。结论成功建立了2型糖尿病db/db小鼠血管内皮细胞原代培养方法,获得的种子细胞可进行血管相关疾病研究。Objective To explore the primary culture method of vascular endothelial cells of diabetic db/db mice and detect its growth curve.Method Vascular endothelial cells were obtained from thoracic aorta and abdominal aorta of type 2 diabetic db/db mice by tissue attachment method.The cells were purified by subculture and their growth curves were detected.Result The vascular endothelial cells were obtained rapidly and efficiently had stable cell characteristics.Conclusion The primary culture method of vascular endothelial cells in type 2 diabetic db/db mice had been successfully established,and the seed cells obtained could be used for the study of vascular related diseases.

关 键 词：糖尿病小鼠 血管内皮细胞 原代培养 

分 类 号：R581[医药卫生—内分泌]