不同等级亳白芍药材及饮片的质量评价研究  

Study on quality evaluation in different grades of medicinal materials and medicinal pieces of Paeonia radix alba in Bozhou

作　　者：李芳 黄卫锋 汪海斌 周建理 黄艳梅 王德胜 LI Fang;HUANG Weifeng;WANG Haibin;ZHOU Jianli;HUANG Yanmei;WANG Desheng(Instrumental Analysis Lab,National Chinese Medicinal Materials Products Quality Supervision and Inspection Center(Anhui),Anhui Province,Bozhou 236800,China;Quality and Technical Department,Bozhou Product Quality Supervision and Testing Institute,Anhui Province,Bozhou 236800,China;Quality Department,Anhui Dechang Pharmaceutical Co.,Ltd.,Anhui Province,Bozhou 236800,China)

机构地区：[1]国家中药材产品质量监督检验中心(安徽)仪器分析室,安徽亳州236800 [2]安徽省亳州市产品质量监督检验所质量技术部,安徽亳州236800 [3]安徽德昌药业股份有限公司质量部,安徽亳州236800

出　　处：《中国医药导报》2021年第24期4-7,共4页China Medical Herald

基　　金：国家自然科学基金青年科学基金项目(81503115);安徽省重点研究与开发计划面上攻关项目(1804a0802226);安徽高校自然科学研究项目(KJ2018A0276);安徽省亳州市重点研发计划项目(bzzc2019010)。

摘　　要：目的探讨不同等级亳白芍药材及饮片的质量差异。方法参照《中华人民共和国药典》(2020年版)一部、四部对不同等级亳白芍药材及饮片进行理化指标分析;采用薄层色谱法对其进行定性鉴别;采用高效液相色谱法对芍药苷含量进行测定,色谱柱为Waters XBridge C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(14∶86),流速为1.0 ml/min,检测波长为230 nm,柱温为30℃。结果所有样品的测定指标(水分、总灰分、水溶性浸出物、二氧化硫残留量)均符合要求。所有样品在与对照品相同位置上均显相同颜色斑点。不同等级的亳白芍药材中芍药苷含量有一定差异,二级品含量最高。不同等级的亳白芍饮片中芍药苷含量差异不大。结论市场上主要以中部直径及片径划分亳白芍药材及饮片规格等级并不能客观反映其内在质量。Objective To explore the quality differences of different grades of Paeonia radix alba in Bozhou.Methods The physical and chemical indexes of Paeonia radix alba in Bozhou were analyzed according to part one and part four of the Pharmacopoeia of the People’s Republic of China(2020 edition);the thin layer chromatography method was used to qualitatively identify the Paeonia radix alba in Bozhou;and the content of paeoniflorin was determined by high performance liquid chromatography method.Waters XBridge C18 column(250 mm×4.6 mm,5μm)was used as the column,acetonitrile-0.1% phosphoric acid solution(14:86)was used as the mobile phase,flow rate was 1.0 ml/min,the detection wavelength was 230 nm,column temperature was 30℃.Results The determination indexes of all samples(moisture,total ash,water-soluble extract,sulfur dioxide residue)were all meet the requirements.All samples showed the same color spots in the same position as the reference.The content of paeoniflorin in different grades of Paeonia radix alba in Bozhou were different,and the content of the second grade was the highest.There was little difference in paeoniflorin content among different grades of Paeonia radix alba in Bozhou.Conclusion In the market,the specification grades of medicinal materials and medicinal pieces of Paeonia radix alba in Bozhou based on the central diameter and piece diameter cannot reflect the intrinsic quality,objectively.

关 键 词：亳白芍 不同等级 芍药苷 质量评价 

分 类 号：R284.1[医药卫生—中药学]