西藏当雄县牦牛巴氏杆菌病血清流行病学调查  

Seroepidemiological Investigation on Pasteurellosis in Yaks in Damxung County of Tibet

作　　者：马弘财 王冬经 元振杰[1] 石斌[1] 曾江勇[1] 德吉[1] Ma Hongcai;Wang Dongjing;Yuan Zhenjie;Shi Bin;Zeng Jiangyong;De Ji(Tibet Livestock Reserch Institution,Tibet Academy of Agriculture and Animal Science,Lhasa,Tibet 850009,China)

机构地区：[1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009

出　　处：《中国动物检疫》2021年第9期17-19,23,共4页China Animal Health Inspection

基　　金：西藏自治区自然科学基金项目(XZ2019ZRG-94)。

摘　　要：为了解西藏当雄县牦牛巴氏杆菌病流行现状,2019—2020年在牦牛养殖较为集中的5个乡镇,采集存栏2000头以上的2个养殖场以及存栏30头以上的56个牧户未免疫牦牛血清1124份,通过酶联免疫吸附试验(ELISA)进行牛巴氏杆菌血清抗体检测,并使用R软件对不同年龄、性别、饲养方式牦牛的检测结果进行统计分析。结果显示:当雄县牦牛巴氏杆菌个体阳性率为11.57%(130/1124),群体阳性率为89.66%(52/58);养殖场牦牛个体阳性率(12.96%)与牧户(10.92%)无统计学差异(P>0.05);公牦牛个体阳性率(10.07%)与母牦牛(12.45%)无统计学差异(P>0.05);3岁以下牦牛个体阳性率(5.08%)与6岁以上牦牛(22.81%)有统计学差异(P<0.01,OR=5.52)。结果表明,巴氏杆菌病在该县牦牛群中流行普遍,无养殖模式和性别差异,但老龄牦牛感染率较高。结果提示,当雄县需要加强牦牛饲养管理和巴氏杆菌疫苗免疫,及时淘汰老龄牦牛。In order to identify the prevalence status of pasteurellosis in yaks in Damxung County of Tibet from 2019 to 2020,1124 serum samples were collected from yaks without vaccination in 56 households with an inventory of above 30 heads and two farms with above 2000 heads in fi ve townships where yaks were intensively kept to detect serum antibodies against Pasteurella bovis by enzyme-linked immunosorbent assay(ELISA).Then,the results for yaks under diff erent ages,genders and feeding modes were summarized and analyzed by R software.The results showed that the individual positive rate of Pasteurella bovis was 11.57%(130/1124)and the rate at the population level was 89.66%(52/58);no statistical diff erence(P>0.05)was found between the individual positive rate in farms(12.96%)and that in households(10.92%),and so were those of male yaks(10.07%)and female yaks(12.45%);the individual positive rate of yaks less than three years old(5.08%)was statistically diff erent(P<0.01,OR=5.52)from those above six years old(22.81%).It was concluded that pasteurellosis was widely prevalent in yaks in the county,without any diff erence in either feeding modes or gender,but the infection rate of aged yaks was relatively high.It was suggested that feeding management and vaccination against pasteurellosis should be strengthened and aged yaks should be eliminated in time.

关 键 词：巴氏杆菌 牦牛 血清流行病学 ELISA 当雄县 

分 类 号：S851.3[农业科学—预防兽医学]