果蝇心脏发育标记基因Lbe抗体的制备与应用  

Preparation and Application of the Antibody for Drosophila Heart Development Marker Gene Lbe

作　　者：赵梦婧 覃彬 陈宇 吴秀山[1] 江志钢[1] 万永奇[1] ZHAO Mengjing;QIN Bin;CHEN Yu;WU Xiushan;JIANG Zhigang;WAN Yongqi(The Center for Heart Development,the College of Life Sciences,Hunan Normal University,Changsha 410081,China)

机构地区：[1]湖南师范大学生命科学学院心脏发育研究中心,长沙410081

出　　处：《激光生物学报》2021年第4期333-338,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(81700338,81470449,81670290);湖南省教育厅优秀青年项目(19B342)。

摘　　要：黑腹果蝇(Drosophila melanogaster)的心脏发育调控基因与人类的相关基因具有高度同源性。Lbe基因是果蝇心脏发育的重要调控基因。为了深入研究Lbe基因在心脏发育中的调控功能,采用DNA免疫技术制备了抗果蝇Lbe蛋白的多克隆抗体。通过PCR扩增Lbe基因序列,将该序列与真核表达载体pCAGGS-P7同源重组,以该同源重组载体免疫4周龄小鼠,获得含有抗Lbe多克隆抗体的血清。蛋白质印迹(Western blot)和胚胎抗体染色结果表明,该抗体的特异性较好。该抗体为随后的Lbe功能的研究奠定了基础。The heart development genes of Drosophila melanogaster are highly conserved with human heart development genes.Lbe(Ladybird early)is one of the important genes involved in Drosophila heart development.In order to further un-derstand the regulatory role of Lbe gene in heart development,the anti-Lbe antibody is prepared with DNA immunization tech-nique.The Lbe coding sequence was amplified with PCR,and then it was homologously recombined with the eukaryotic expres-sion vector pCAGGS-P7.This homologous recombinant vector was used as antigen to immunize four weeks old mice to obtain the serum containing anti-Lbe polyclonal antibody.Western blot and embryo antibody staining results showed that the specificity of the antibody was good.The antibody provides a foundation for the subsequent study of function of Lbe.

关 键 词：果蝇 心脏发育 Lbe DNA免疫 多克隆抗体 

分 类 号：Q78[生物学—分子生物学]