新疆石河子地区哈萨克族、回族、汉族人群20个STR基因座的遗传多态性研究  

Genetic Polymorphism of 20 STR Loci in Kazakh,Hui and Han Population in Shihezi Region of Xinjiang

作　　者：杨秀[1] 牟睿 曹雨薇[1] 王郗 李春香[1] 蔡君成 刘焕杰[1] 马温华[2,3] YANG Xiu;MU Rui;CAO Yuwei;WANG Xi;LI Chunxiang;CAI Juncheng;LIU Huanjie;MA Wenhua(Shihezi Public Security Bureau of Xinjiang,Xinjiang 832000,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038;Beijing Engineering Research Center of Crime Scene Evidence Examination,Beijing 100038,China)

机构地区：[1]新疆石河子市公安局,新疆832000 [2]公安部物证鉴定中心,北京100038 [3]北京市现场物证检验工程技术研究中心,北京100038

出　　处：《激光生物学报》2021年第4期339-347,共9页Acta Laser Biology Sinica

基　　金：公安部物证鉴定中心北京市现场物证检验工程技术研究中心开放课题项目(2018CSEEKFKT02)。

摘　　要：为了调查新疆石河子地区哈萨克族、回族、汉族群体20个STR基因座遗传多态性,研究其法医学应用价值,用DNATyper21TM荧光复合扩增试剂盒对3个群体的20个STR基因座进行扩增,并用3500xL基因分析仪对其进行检测。用PowerStats v12、Arlequin v3.5、Phylip-3.695等软件进行统计计算,并对群体间遗传距离进行比较。研究结果表明:哈萨克族共检出231个等位基因,基因频率(F)分布在0.0008~0.5008之间,杂合度(H)在0.669~0.923之间,个体识别能力(DP)在0.817~0.988之间,多态信息含量(PIC)在0.60~0.92之间,非父排除概率(PE)在0.380~0.843之间,累积三联体非父排除率(CPEtri)为0.999999999,累积二联体非父排除率(CPEduo)为0.999998212,累积个体识别率(TDP)为1-7.60×10–26;回族共检出237个等位基因,F分布在0.0008~0.5334之间,H在0.833~0.928之间,DP在0.949~0.986之间,PIC在0.81~0.91之间,PE在0.661~0.853之间,CPEtri为0.999999996,CPEduo为0.999996725,TDP为1-4.77×10–25;汉族共检出253个等位基因,F在0.0008~0.5410之间,H在0.829~0.943之间,DP在0.958~0.986之间,PIC在0.83~0.91之间,PE在0.655~0.884之间,CPEtri为0.999999996,CPEduo为0.999995459,TDP为1-1.15×10–24。通过Phylip-3.695软件分析可知,新疆石河子地区哈萨克族人群与汉族、回族人群间的遗传距离远,汉族与回族群体遗传距离近。本文研究结果为新疆石河子地区这3个民族的群体遗传学和法医学应用提供了基础数据支持。The aim of this study was to determine the frequencies and parameters of 20 STR loci among Kazakh,Hui and Han population in Shihezi area of Xinjiang.The 20 STR loci of unrelated individuals of three population in Shihezi were am-plified by DNATyperTM21 Kit,and the amplification products were analyzed through 3500xL genetic analyzer.Genetic poly-morphism data and genetic distance were analyzed by PowerStats v12,Arlequin v3.5,Phylip-3.695 software.231 alleles were identified in Kazakh population with their F ranging from 0.0008~0.5008.Heterozygosity(H)ranged among 0.669~0.923,discriminant power(DP)ranged among 0.817~0.988,polymorphism information content(PIC)ranged among 0.60~0.92,and paternity exclusion(PE)ranged among 0.380~0.843,comulative probability of exclusion presented 0.999999999 in triplet setting(CEPtri)and 0.999998212 in duo sttuations(CEPduo),total probability of discrimination power(TDP)was within 1-7.60×10-26;237 alleles were identified in Hui population with their F ranging from 0.0008~0.5334.H ranged among 0.833~0.928,DP ranged among 0.949~0.986,PIC ranged among 0.81~0.91,and PE ranged among 0.661~0.853,comulative probability of exclusion presented 0.999999996 in CEPtri and 0.999996725 in CEPduo,TDP was within 1-4.77×10-25;253 alleles were identified in Han population with their F ranging from 0.0008~0.5410.H ranged among 0.829~0.943,DP ranged among 0.958~0.986,PIC ranged among 0.83~0.91,and PE ranged among 0.655~0.884,comu-lative probability of exclusion presented 0.999999996 in CEPtri and 0.999995459 in CEPduo,TDP was within 1-1.15×10-24.The genetic distance was calculated by Phylip-3.695 softwarit,and was relatively far apart between Kazakh and Hui,Han,whereas relatively close between Han and Hui.The results of this work provided reference data for human population genetics and forensic DNA research in this area.

关 键 词：法医遗传学 STR基因座 遗传多态性 基因频率 新疆石河子地区 

分 类 号：Q987[生物学—遗传学]