补肾壮骨颗粒对去势大鼠子宫内膜及ERα、ERβ、GPCR30的影响  

Effect of Bushen Zhuanggu Granule on endometrium and ERα, ERβ and GPCR30 in ovariectomized rats

作　　者：袁秋虹 罗骏 李娟敏 彭琪媛 邓伟民 YUAN Qiuhong;LUO Jun;LI Juanmin;PENG Qiyuan;DENG Weimin(Guangdong Pharmaceutical University,Guangzhou 510010,China;Chinese People’s Liberation Army General Hospital of Southern War Zone,Guangzhou 510010,China;Guangzhou University of Chinese Medicine,Guangzhou 510010,China;The Third Affiliated Hospital of Southern Medical University,Guangzhou 510010,China;Foresea Life Insurance Guangzhou General Hospital,Guangzhou 511300,China)

机构地区：[1]广东药科大学,广东广州510010 [2]南部战区总医院,广东广州510010 [3]广州中医药大学,广东广州510010 [4]南方医科大学第三附属医院,广东广州510010 [5]前海人寿广州总医院,广东广州511300

出　　处：《中国骨质疏松杂志》2021年第8期1117-1121,1128,共6页Chinese Journal of Osteoporosis

基　　金：省部级应用基础研究项目(16BJZ57);天河区科技计划项目(201704KW029)。

摘　　要：目的研究补肾壮骨颗粒治疗绝经后骨质疏松症期间对子宫内膜及ERα、ERβ、GPCR30的影响。方法将60只6月龄SPF级SD雌性大鼠随机分为模型组、雷洛昔芬组、戊酸雌二醇组、中药组、假手术组,去卵巢建立绝经后骨质疏松症模型或行假手术。各组用药干预3个月后随机抽取半数测量骨密度,运用HE染色观察子宫组织形态结构并测量子宫内膜厚度、直径,通过Western blot检测ERα、ERβ、GPCR30的表达量。余下半数大鼠继续用药干预3个月(总用药6个月)并同样用上述方法处理检测。结果中药组股骨及全身骨密度高于模型组,比较差异有统计学意义(P<0.05),子宫内膜厚度及直径大于模型组,但仅用药6个月的直径与MG的差异有统计学意义(P<0.05),ERα、ERβ蛋白相对表达量高于模型组,差异有统计学意义(P<0.05),GPCR30蛋白相对表达量高于模型组,差异无统计学意义(P>0.05)。结论补肾壮骨颗粒可以改善绝经后骨质疏松症导致的低骨量,同时子宫内膜无明显不良反应。Objective To study the effect of Bushen Zhuanggu Granule on the expression of endometrium and ERα,ERβand GPCR30 in postmenopausal osteoporosis.Methods Sixty female Sprague-Dawley rats of 6 months old were randomly divided into sham operation group,model group,raloxifene group,estradiol valerate group and traditional Chinese medicine group.The rats in each group except sham operation group were established as postmenopausal osteoporosis model,and treated with drug intervention for 3 months,half of the rats in each group was randomly selected for checking the bone density,and the uterus tissue was preserved for HE to detect the endometrial thickness and diameter and for western blot that detect the expression of ERα,ERβand GPCR30.The remaining half of the rats continued to use the drug for 3 months,at the end,treated with the same method above.Re sults The femur and whole-body bone density of the Chinese medicine group were higher than that of the model group,the difference was statistically significant(P<0.05).The endometrial thickness and diameter were larger than the model group,but only the difference in diameter after 6 months of treatment was statistically significant(P<0.05).The relative expression of ERαand ERβprotein was higher than that of the model group(P<0.05).There was no significant difference between the relative expression of GPCR30 protein and the model group(P>0.05).Conclusion Bushen Zhuanggu Granule can improve the lowbone mass caused by postmenopausal osteoporosis and has no obvious adverse effects on the endometrium.

关 键 词：绝经后骨质疏松症 补肾壮骨颗粒 雌激素受体Α 雌激素受体Β G蛋白偶联雌激素受体30 

分 类 号：R28[医药卫生—中药学]