sox-9通过Wnt3a/β-catenin通路促进大鼠骨髓间充质干细胞分化  

Sox-9 promotes differentiation of rat bone marrow mesenchymal stem cells by inhibiting Wnt3a/β-catenin pathway

作　　者：樊薰勤 张明勇[1] 李雯婷[1] 钱雪丰[1] 徐宁路[1] 王勇[1] 曹平[1] 张杰[2] 郭奉斌[1] 赵志强[1] 郑金鹏 刘盾 刘拴[1] FAN Xunqin;ZHANG Mingyong;LI Wenting;QIAN Xuefeng;XU Ninglu;WANG Yong;CAO Ping;ZHANG Jie;GUO Fengbin;ZHAO Zhiqiang;ZHENG Jinpeng;LIU Dun;LIU Shuan(Department of Orthopedics,Tianyou Hospital Affiliated to Wuhan University of Science&Technology,Wuhan 430064;Department of Interventional Radiography,Tianyou Hospital Affiliated to Wuhan University of Science&Technology,Wuhan 430064,China)

机构地区：[1]武汉科技大学附属天佑医院骨科,湖北武汉430064 [2]武汉科技大学附属天佑医院放射介入科,湖北武汉430064

出　　处：《中国骨质疏松杂志》2021年第8期1169-1173,共5页Chinese Journal of Osteoporosis

基　　金：湖北省卫生计生委面上项目(WJ2017M171)。

摘　　要：目的探讨sox-9对骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,BMSCs)增殖能力和分化能力的影响。方法分离并鉴定BMSCs,对细胞进行sox-9过表达和敲降处理。将细胞分为对照组、过表达对照组、干扰对照组、sox-9过表达组、sox-9干扰组。CCK8法检测细胞增殖活性;免疫组化观察细胞的Ⅱ型胶原蛋白(CollageⅡ)和聚蛋白聚糖(Aggrecan)表达水平;Western blot检测细胞sox-9、Wnt3a、β-连环蛋白(β-catenin)表达水平。结果各组细胞增殖能力无显著差异(P>0.05);sox-9过表达组的CollageⅡ和Aggrecan表达显著升高,而Wnt3a和β-catenin的表达显著降低(P<0.05)。结论sox-9可能是调控间质干细胞向软骨细胞分化能力的关键因子,通过抑制Wnt3a/β-catenin信号途径,促进其向软骨细胞分化。Objective To investigate the effect of Sox-9 on the proliferation and differentiation of bone marrowmesenchymal stem cells(BMSCs).Methods BMSCs were isolated and identified.Sox-9 was over-expressed or knocked down in the cells.The cells were divided into blank control group,over-expression control group,interference control group,Sox-9 over-expression group,and Sox-9 interference group.MTT method was used to measure the cell growth.Immunohistochemical method was used to detect the expressions of collageⅡand aggrecan.Western blotting was used to detect the expressions of Sox-9,Wnt3a,andβ-catenin.Re sults There was no significant difference in cell proliferation among the groups(P>0.05).The expression of collageⅡand aggrecan was significantly increased in Sox-9 over-expression group,while the expression of Wnt3a andβ-catenin was significantly decreased(P<0.05).Conclusion Sox-9 may be a key factor in regulating the differentiation ability of BMSCs to chondrocytes.It promotes the differentiation of BMSCs to chondrocytes by inhibiting Wnt3a/β-catenin signaling pathway.

关 键 词：骨髓间充质干细胞 Wnt3a/β-catenin sox-9 软骨分化能力 大鼠 

分 类 号：R681.3[医药卫生—骨科学] R-332[医药卫生—外科学]