应用基因芯片技术研究冠心病秽浊痰阻证差异表达基因及其通路  

Study on Differentially Expressed Genes and Pathways of Coronary Heart Disease with Turbid Phlegm Obstruction Syndrome Using Gene Chip Technology

作　　者：翟雪芹[1] 何义 高玉[1] 陈战西[1] 王晶[3] 朱小莉[1] 周云[1] 王晓峰[1] ZHAI Xue-qin;HE Yi;GAO Yu;CHEN Zhan-xi;WANG Jing;ZHU Xiao-li;ZHOU Yun;WANG Xiao-feng(Fourth Department of Cardiology,Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Urumqi,830000;Department of Cardiac Intensive Care,Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Urumqi,830000;Central Experiment,Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Urumqi,830000)

机构地区：[1]新疆医科大学附属中医医院心内四科,乌鲁木齐830000 [2]新疆医科大学附属中医医院心脏重症监护室,乌鲁木齐830000 [3]新疆医科大学附属中医医院中心实验室,乌鲁木齐830000

出　　处：《中国中西医结合杂志》2021年第8期922-927,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81760794)。

摘　　要：目的探索冠状动脉粥样硬化性心脏病(CHD)秽浊痰阻证患者差异表达基因及相关信号通路,从分子水平阐明CHD秽浊痰阻证的生物学特点。方法纳入2015年2月—2016年3月就诊于新疆医科大学附属中医医院明确诊断为CHD的患者24例,其中秽浊痰阻证组12例、非秽浊痰阻证组12例,另纳入经冠脉造影术排除CHD的12例受试者为对照组。采集外周血并提取总的RNA,通过Human GenomeU133Plus2.0芯片检测获取基因,使用R语言limma软件包筛选差异表达基因,利用STRING构建差异基因编码蛋白互作网络(PPIs),应用Cytoscape插件识别差异表达基因的核心模块,应用Clusterprofiler软件包对差异表达基因进行GO和KEGG功能富集分析。结果与对照组比较,CHD患者共筛选出184个差异表达基因,其中165个差异基因表达上调,19个差异基因表达下调。CHD秽浊痰阻证组与非秽浊痰阻证组差异表达基因42个,其中34个差异基因表达上调,8个差异基因表达显著下调,GO和KEGG富集分析中,7个模块显著富集了固有免疫调节、固有免疫正向调节、固有免疫的激活和Fc受体信号通路,模块基因参与了MAPK、NF-κB、PI3K/Akt等信号通路、Ⅰ型糖尿病相关通路、C-型凝集素受体信号通路、剪切应力与动脉粥样硬化相关通路等与CHD有密切关联的信号通路。结论 CHD秽浊痰阻证与冠心病非秽浊痰阻证患者存在显著的差异表达基因,这些差异表达基因显著富集在与冠心病相关的多条功能通路中。Objective To explore the differentially expressed genes and related signaling pathways in patients with coronary heart disease(CHD), and to clarify the biological characteristics of CHD with turbid phlegm obstruction syndrome(TPOS) from the molecular level.Methods Twenty-four patients with CHD who were admitted to the Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University from February 2015 to March 2016 were included, including 12 patients in the group of TPOS and 12 patients in the group of non TPOS.Meanwhile 12 subjects who were excluded from CHD by coronary angiography as the control group.The peripheral blood was collected and total RNA was extracted. The data were detected by Human Genome U133 Plus2.0 chip. The differentially expressed genes were screened by Limma software package in R language, and the protein-protein interaction networks(PPIs) was constructed by STRING.Cytoscape screened the core modules of differentially expressed genes.Clusterprofiler software package was used for GO and KEGG enrichment analysis of differentially expressed genes.Results Compared with the control group, a total of 184 differentially expressed genes were screened in CHD patients, among which 165 differentially expressed genes were up-regulated and 19 differentially expressed genes were down-regulated.There were 42 differentially expressed genes between the TPOS group and the non-TPOS group, among which 34 differentially expressed genes were up-regulated and 8 differentially expressed genes were significantly down-regulated.Seven module genes significantly enriched regulation of innate immune response, positive regulation of innate immune response, activation of innate immune response, and FC receptor signaling pathway.Module genes were significantly involved in MAPK/NF-κB/PI3 K-Akt/TNF, Type Ⅰ diabetes mellitus, C-type lectin receptor signaling pathway and fluid shear stress and atherosclerosis signaling pathways related to CHD.Conclusion There are significant differentially expressed genes

关 键 词：冠心病 秽浊痰阻证 差异表达基因 

分 类 号：R541.4[医药卫生—心血管疾病] Q811.4[医药卫生—内科学]