miR-142-3p调控雌激素受体1在妊娠期糖尿病并发子痫前期中的作用机制  

Study on the mechanism of miR-142-3p regulation of estrogen receptor 1 in gestational diabetes mellitus with preeclampsia

作　　者：任国华 石云[1] 张琳琳[1] 陈英[1] Ren Guohua;Shi Yun;Zhang Linlin;Chen Ying(Department of Obstetrics and Gynecology,Linyi People’s Hospital,Linyi 276003,China)

机构地区：[1]山东省临沂市人民医院妇产科,276003

出　　处：《中华内分泌外科杂志》2021年第4期433-437,共5页Chinese Journal of Endocrine Surgery

基　　金：山东省医药卫生科技发展计划项目(2014WS0431);中国博士后基金面上项目(2012M521356)。

摘　　要：目的探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)并发子痫前期(preeclampsia,PE)孕妇血清和胎盘组织中的miR-142-3p和雌激素受体1(estrogen receptor,ER1)在疾病发生、发展中的意义及调控机制。方法选择2019年6月至2020年6月在临沂市人民医院产科就诊的198位孕妇作为研究对象,包括66例GDM孕妇(GDM组),60例GDM并发PE孕妇(GDM+PE组)和72例正常孕妇(对照组)。检测临床指标,收集妊娠结局资料;采用定量实时聚合酶联反应法测定研究对象血清和胎盘组织中miR-142-3p和ER1 mRNA的相对表达量;采用western blot法检测胎盘组织中的ER1蛋白表达水平;使用miR-142-3p对人绒毛膜滋养层细胞HTR-8/SVneo处理。结果GDM+PE组的血清和胎盘组织中miR-142-3p表达量显著低于GDM组和对照组(P均<0.05);GDM+PE组的血清和胎盘组织中ER1 mRNA表达量显著高于GDM组和对照组(P均<0.05)。GDM+PE组孕妇的血清和胎盘组织中miR-142-3p和ER1 mRNA相对表达量存在显著负相关关系(r=-0.589和-0.643,P=0.006和<0.001)。使用miR-142-3p对HTR-8/SVneo细胞转染后,mimic组的ER1 mRNA表达量为(1.09±0.14),显著低于mimic NC组(2.14±0.52)、inhibitor组(3.69±0.88)和inhibitor NC组(2.26±0.43)的表达量(P<0.001),而inhibitor组的DNMT1表达量均显著高于其他3组(P<0.001)。结论GDM并发PE患者miR-142-3p水平降低,调控ER1水平上升,参与到疾病的发生、发展中。Objective To investigate the significance and regulatory mechanism of miR-142-3p and ER1 in serum and placenta of pregnant women with gestational diabetes mellitus(GDM)complicated with preeclampsia(PE)in the occurrence and development of disease.Methods A total of 198 pregnant women admitted from Jun.2019 to Jun.2020 were selected as the study subjects,including 66 pregnant women with GDM(GDM group),60 pregnant women with GDM complicated with PE(GDM+PE group)and 72 normal pregnant women(control group).Clinical indicators were detected and pregnancy outcome data were collected.The relative expression levels of miR-142-3p and ER1 mRNA in serum and placental tissues of study subjects were determined by quantitative real-time polymerase link assay.The expression of ER1 protein in the placenta was detected by Western blot.Human choriotrophoblast cells HTR-8/SVneo were treated with miR-142-3p.Results The expression levels of miR-142-3p in serum and placenta tissues in GDM+PE group were significantly lower than those in GDM+PE group and control group(P<0.05).The mRNA expression of ER1 in serum and placenta in GDM+PE group was significantly higher than that in GDM+PE group and control group(P<0.05).There was a significant negative correlation between the relative expression levels of miR-142-3p and ER1 mRNA in serum and placental tissues of pregnant women in the GDM+PE group(r=-0.589 and-0.643,P=0.006 and<0.001).After transfection of HTR-8/SVneo cells with miR-142-3p,ER1 mRNA expression in the mimic group was 1.09±0.14,significantly lower than that of NC group(2.14±0.52),inhibitor group(3.69±0.88)and inhibitor NC group(2.26±0.43)(P<0.001).The expression of DNMT1 in inhibitor group was significantly higher than that in the other three groups(P<0.001).Conclusion In patients with GDM complicated with PE,miR-142-3p levels are reduced and ER1 levels are increased,which may be involved in the occurrence and progression of the disease.

关 键 词：妊娠期糖尿病 子痫前期 miR-142-3p 雌激素受体1 

分 类 号：R587.1[医药卫生—内分泌]