赣州市2013年—2019年麻疹病毒野毒株与疫苗株的快速鉴别  

Rapid identification of measles virus wild strains and vaccine strains in Ganzhou City from 2013 to 2019

作　　者：熊衍峰[1] 陈祥发[1] 李健雄[2] 谭慧琳 李建华[1] 胡晓军[1] XIONG Yan-feng;CHEN Xiang-fa;LI Jian-xiong;TAN Hui-lin;LI Jian-hua;HU Xiao-jun(Ganzhou Municipal Center for Disease Control and Prevention,Ganzhou,Jiangxi 341000,China;不详)

机构地区：[1]赣州市疾病预防控制中心,江西赣州341000 [2]江西省疾病预防控制中心,江西南昌330029

出　　处：《中国卫生检验杂志》2021年第14期1670-1672,共3页Chinese Journal of Health Laboratory Technology

基　　金：江西省卫生计生委科技计划(20176043)。

摘　　要：目的利用反转录-聚合酶链反应-限制性片段长度多态性分析(RT-PCR-RFLP)技术,建立简便、快速鉴别赣州市2013年—2019年麻疹病毒野毒株和疫苗株的方法。方法用Vero-SLAM细胞对疑似麻疹病例咽拭子标本进行病毒分离培养,并对分离的26株麻疹毒株提取的核酸采用RT-PCR-RFLP方法进行麻疹病毒鉴定。根据是否含有AflⅡ限制性内切酶的酶切位点鉴别野毒株和疫苗株,同时对所有毒株进行H基因全长序列测定,验证RT-PCR-RFLP方法。结果26株麻疹毒株酶切后均呈单一条带,不能被AflⅡ限制性内切酶切开。26株毒株H基因序列测定结果7666 bp~7671 bp均不含AflⅡ限制性内切酶位点。结论采用RT-PCR-RFLP方法鉴别赣州市麻疹病毒野毒株与疫苗株,快速、简便、可行。Objective A simple and rapid method for the identification of wild measles virus strains and vaccine strains from 2013 to 2019 in Ganzhou City was established by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism(RT-PCR-RFLP).Methods Vero-SLAM cells were used to isolate and culture the virus from throat swabs of suspected measles cases,and the nucleic acid extracted from 26 isolated measles strains was identified by RT-PCR-RFLP method.The wild strains and vaccine strains were identified according to whether they contained AflⅡrestriction enzyme restriction sites,and the full-length H gene sequence of all strains was determined to verify the RT-PCR-RFLP method.Results All the 26 measles strains showed a single band and could not be cut by AflⅡrestriction enzyme.The H gene sequence of 26 virulent strains was 7666bp-7671bp without AflⅡrestriction enzyme site.Conclusion RT-PCR-RFLP is a rapid and simple method for identifying vaccine strain and wild strain of measles virus.

关 键 词：麻疹病毒 野毒株 疫苗株 快速鉴别 

分 类 号：R446[医药卫生—诊断学]