中国辣椒低温响应转录因子CBF全基因组鉴定与分析  

Genome-wide Identification and Analysis of the Cold Responsive Transcription factor(CBF)in Capsicum chinense

作　　者：叶正 高崇伦 Zakaria Gagoshidze 舒黄英 包文龙 朱婕[1] 成善汉[1] 朱国鹏[1] 汪志伟[1] Ye Zheng;Gao Chonglun;Zakaria Gagoshidze;Shu Huangying;Bao Wenlong;Zhu Jie;Cheng Shanhan;Zhu Guopeng;Wang Zhiwei(Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,College of Horticulture,Hainan University,Haikou,570228)

机构地区：[1]海南大学园艺学院,海南省热带园艺作物品质调控重点实验室,海口570228

出　　处：《分子植物育种》2021年第15期4903-4910,共8页Molecular Plant Breeding

基　　金：国家重点研发计划(2018YFD1000800);海南省研究生创新基金(Hyb 2017-18);海南大学高层次人才启动基金(KYQD1656)共同资助。

摘　　要：CBF(C-repeat binding factor)转录因子在植物应对低温等非生物胁迫和提高耐寒性中发挥着重要作用。为了探究中国辣椒(Capsicum chinense Jacq.)CBF基因家族的基本特性,利用生物信息学方法,在中国辣椒基因组中鉴定获得8个CBF基因家族转录因子,对其理化性质、蛋白结构、系统发育、作用元件和基因转录进行了分析。结果表明,8个CBF蛋白长度为156~290 aa,蛋白分子量为17.75~32.21 kD;这些蛋白均包含AP2结构域。系统发育分析表明,中国辣椒8个CBF基因可分为两个亚组,其中A组包含7个基因,B组仅包含1个基因。表达分析表明,8个CBF基因对低温冷胁迫均有不同程度的响应。研究结果概括了中国辣椒CBF基因家族的基本特性,为CBF转录因子的深入研究和应用积累了基础数据。CBF(C-repeat binding factor)transcription factors play an important role in responses to abiotic stresses such as low temperature.In order to explore the basic characteristics of the Capsicum chinense CBF gene family,using bioinformatics methods,eight CBF gene family transcription factors were identified in the Capsicum chinense genome.The physicochemical properties,protein structure,phylogeny,cis-elements and gene transcription were analyzed.The results showed that the eight CBF proteins are 156~290 aa in length and 17.75~32.21 kD in molecular weight,each of which contains AP2 domain.Phylogenetic tree analysis suggested that eight CBF genes could be divided into two subgroups.Group A contains 7 genes,and group B contains only 1 gene.Transcriptional analysis showed that the eight CBF genes were responsive to cold stress.The research results summarized the basic characteristics of the Capsicum chinense CBF gene family,and provide references for the in-depth research and application of CBF transcription factors.

关 键 词：中国辣椒(Capsicum chinense) 低温响应 CBF转录因子 基因家族 

分 类 号：S641.3[农业科学—蔬菜学]