黄瓜CsPI基因的克隆及不同器官中的表达  

Cloning of CsPI Gene and Its Expression in Different Organs of Cucumber

作　　者：张微微[1] 佘为为 徐菲[1] 周鹏[1] 高东菊[1] 成文竞[1] Zhang Weiwei;She Weiwei;Xu Fei;Zhou Peng;Gao Dongju;Cheng Wenjing(Department of Plant Science and Technology,Shanghai Vocational College of Agriculture and Forestry,Shanghai,201699)

机构地区：[1]上海农林职业技术学院植物科学技术系,上海201699

出　　处：《分子植物育种》2021年第15期4920-4925,共6页Molecular Plant Breeding

基　　金：上海市“科技创新行动计划”农业领域项目(20392001300);上海自然科学基金(20ZR1439600);上海农林职业技术学院中青年领军人才项目(A2-0273-20-01-16);校内课题项目(KY2-0000-20-01)共同资助。

摘　　要：MADS-box家族基因在植物花器官发育中具有重要的作用。本研究采用同源克隆技术,从黄瓜(Cucumis sativus L.)顶芽中克隆获得B类MADS-box基因CsPI的cDNA序列。序列分析表明,CsPI基因cDNA序列的完整开放阅读框(ORF)为636 bp,编码211个氨基酸,分子质量为24904,理论等电点为8.42。蛋白质序列相似性比对和分子系统发生分析表明,CsPI聚类于PI进化支,其C末端包含保守的PI基序。葫芦科PI同源蛋白序列比较保守,其中CsPI与甜瓜的同源性最高,为98.6%。实时荧光定量PCR显示,CsPI基因主要在花器官中的花瓣和雄蕊中表达。本研究为探析黄瓜花器官发育提供了一定理论基础。MADS-box family genes play an important role in the development of plant floral organs.The cDNA sequence of B-class MADS-box gene CsPI involved in flower development was isolated from the apical bud of cucumber(Cucumis sativus L.)by homology cloning.The open reading frame(ORF)of CsPI was 636 bp,encoding 211 amino acids.Molecular weight of CsPI was 24904,and the theoretical isoelectric point was 8.42.The molecular phylogeny analysis and protein sequence alignment suggested that CsPI was grouped with the PI lineage,and C domain of CsPI contained a conserved PI motif.Sequences of cucurbitaceae PI homologous proteins were relatively conservative.Among them,CsPI had the highest homology with melon CmPI,which was 98.6%.Quantitative real-time PCR showed that the CsPI gene was mainly expressed in petals and stamens of floral organs.This study provides a theoretical basis for studying the development of cucumber floral organs.

关 键 词：黄瓜 PISTILLATA(PI) 基因 克隆 花 

分 类 号：S642.2[农业科学—蔬菜学]