MYB1和MYB10基因在部分苹果属植物中的变异分析  

Analysis of Variation in MYB1 and MYB10 in Several Malus Plants

作　　者：马超 卜轶凡 张丞慧 马洪峥 胡宝全 Ma Chao;Bu Yifan;Zhang Chenghui;Ma Hongzheng;Hu Baoquan(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin,300392)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《分子植物育种》2021年第15期4935-4943,共9页Molecular Plant Breeding

基　　金：国家自然科学基金(3180011064);天津市高等学校大学生创新创业训练计划项目(201710061038)共同资助。

摘　　要：为研究参与花青苷合成调控MYB1和其等位基因MYB10在苹果属中的多态性和进化特征,以苹果属部分植物为材料,通过RT-PCR技术扩增MYB1和MYB10基因序列,测序并预测分析比较其蛋白质结构。试验共得到6种材料的MYB1序列,除美丽海棠和新疆野苹果基因CDS编码区为1209 bp外,其余均为1239 bp;获得9种材料的MYB10序列,除西府海棠的基因CDS编码区为717 bp外,其余均为732 bp。MYB1与MYB10蛋白序列比较结果显示,MYB1的多态性显著高于MYB10,序列保守性差异可能与其基因功能分化有关,且MYB1有两处多态性位点是碱性的精氨酸和非极性的亮氨酸的互换,且位点恰好位于功能结构域,可能对苹果属不同植物的MYB1功能产生影响。结果表明MYB1和其等位基因MYB10在苹果属中的多态性存在显著差异,揭示了其在进化中产生的功能分化,可为该基因座基因功能研究和苹果果色的调控机制研究提供进一步的理论基础。For the study of the polymorphism and evolutionary characteristics of MYB1 and its allele MYB10 involved in the regulation of anthocyanin biosynthesis in genus Malus.The MYB1 and MYB10 gene sequences amplified by RT-PCR technology in several Malus plants were sequenced and predicted for their protein structures to detect the differences.MYB1 sequences of 6 materials were obtained and the full-length CDS were 1239 bp except for those of Malus cv.Eleyi and Malus sieversii(1209 bp).While,MYB10 sequences of 9 materials were obtained and the full-length CDS was 732 bp(Malus micromalus was 717 bp).The result from the comparison of protein sequences of MYB1 and MYB10 showed that the polymorphismofMYB1 is significantly higher thanMYB10 and the polymorphism difference between the alleles may be related to the differentiation of gene function.Two polymorphism sites,the interchange of alkaline arginine and non-polar leucine,are located on functional domain which may affect the function of MYB1 in different Malus plants.The result suggested that sequence conservatism of the allele MYB10 and MYB1 significantly differed from each other,and the genetic function responsible for evolution is differentiated.The result can provide further theoretical basis for the study of the gene function and regulation mechanism of apple fruit color.

关 键 词：苹果属 MYB1 MYB10 花青苷 转录因子 

分 类 号：S661.1[农业科学—果树学]