瓜蒌薤白半夏汤加味对慢性萎缩性胃炎大鼠的作用机制研究  

Effect and mechanism of modified Gualou Xiebai Banxia Decoction on chronic atrophic gastritis in rats

作　　者：李一芳 查安生[2] 胡建鹏 王睿[2] 汪悦[2] LI Yi-fang;ZHA An-sheng;HU Jian-peng;WANG Rui;WANG Yue(College of Nursing,Anhui University of Chinese Medicine,Hefei 230012,Anhui Province,China;Department of Gastroenterology,First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061,Anhui Province,China;Graduate School,Anhui University of Chinese Medicine,Hefei 230012,Anhui Province,China)

机构地区：[1]安徽中医药大学护理学院,安徽合肥230012 [2]安徽中医药大学第一附属医院脾胃病科,安徽合肥230061 [3]安徽中医药大学研究生院,安徽合肥230012

出　　处：《中国临床药理学杂志》2021年第16期2201-2205,共5页The Chinese Journal of Clinical Pharmacology

基　　金：安徽省自然科学基金青年基金资助项目(1808085QH257);安徽省卫生计生委中医药科研课题基金资助项目(2016zy68);安徽省高校自然科学研究基金资助项目(KJ2020A0445)。

摘　　要：目的探讨瓜蒌薤白半夏汤加味对慢性萎缩性胃炎模型大鼠蛋白酪氨酸激酶2/信号传导与转录激活因子3(JAK2/STAT3)及刺猬(Hhh)信号通路的调控及其对炎症的控制作用。方法用随机数字表法将SD大鼠分为5组:正常组、模型组、对照组和低、高2个剂量实验组,每组12只。对照组灌胃温胃舒胶囊生药液7.5 mg·g^(-1),低、高2个剂量实验组灌胃瓜蒌薤白半夏汤生药液12,48 mg·g^(-1)。正常组和模型组予生理盐水,连续给药4周。用N-甲基-N-硝基-N-亚硝基胍法建立慢性萎缩性胃炎大鼠模型。酶联免疫吸附法检测细胞上清液中胃泌素-17(G-17)含量,蛋白质印迹法检测胃粘膜JAK2、STAT3和Hh信号通路相关音猬因子(Shh)的蛋白表达水平(灰度值),实时荧光定量-PCR法检测上述蛋白的基因表达水平(2~(-△△Ct)值)。结果正常组、模型组、对照组和低、高2个剂量实验组G-17含量分别为(10.12±1.41),(55.70±8.72),(35.76±10.52),(37.38±8.96)和(19.18±9.15)ng·L^(-1);这5组JAK2蛋白相对表达量为0.20±0.04,0.79±0.08,0.28±0.02,0.56±0.08和0.55±0.04;STAT3蛋白相对表达量为0.16±0.03,0.62±0.11,0.21±0.06,0.49±0.03和0.51±0.05;Shh蛋白相对表达量为0.52±0.03,0.13±0.02,0.20±0.02,0.37±0.07和0.38±0.05。基因结果的趋势与蛋白一致。上述指标:模型组与正常组比较,差异均有统计学意义(均P<0.05);低、高2个剂量实验组和对照组与模型组比较,差异均有统计学意义(均P<0.05)。结论瓜蒌薤白半夏汤加味可能通过抑制JAK2/STAT3通路表达、促进Hh信号通路表达起到对抗慢性萎缩性胃炎大鼠的炎症活动的作用。Objective To analyze the regulation of modified Gualou Xiebai Banxia Decoction on protein tyrosine kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)and Sonic Hedgehog(Shh)signaling pathway in chronic atrophic gastritis model rats and its role in controlling inflammatory activity.Methods The SD rats were divided into five groups according to random number table:normal group,model group,control group,experimental-L group,experimental-H group,with 12 rats in each group.The control group was given Wenweishu capsule 7.5 mg·g^(-1),and experimental-L group,experimental-H were clystered respectively Gualou Xiebai Banxia Decoction 12,48 mg·g^(-1).Normal group and model group were given normal saline for 4 weeks.The chronic atrophic gastritis rat model was established by the method of N-methyl-N-nitro-N-nitrosoguanidine.The levels of gastrin-17(G-17)were detected by enzyme-linked immunosorbent assay.The proteins expression(gray value)of JAK2/STAT3 and Shh,transmembrane receptor(Ptch)and related to Hh signaling pathway were detected by Western blotting.The mRNA expressions(2-△△Ct value)of these proteins were detected by real-time PCR.Results G-17 content in normal group,model group,control group,experimental-L group and experimental-H group were(10.12±1.41),(55.70±8.72),(35.76±10.52),(37.38±8.96),(19.18±9.15)ng·L^(-1),respectively;the relative expression of JAK2 protein in the five groups were 0.20±0.04,0.79±0.08,0.28±0.02,0.56±0.08,0.55±0.04,respectively;the relative expression of STAT3 protein were 0.16±0.03,0.62±0.11,0.21±0.06,0.49±0.03,0.51±0.05,respectively;the relative expression of Shh protein were 0.52±0.03,0.13±0.02,0.20±0.02,0.37±0.07,0.38±0.05.The trend of gene results is consistent with that of protein.The differences of the above indexes between the model group and the normal group were statistically significant(all P<0.05);compared with model group,experimental-L group,experimental-H group and control group had statistical significance(all P<0.05).Conclusion Mod

关 键 词：瓜蒌薤白半夏汤 慢性萎缩性胃炎 胃阳虚 蛋白酪氨酸激酶2/信号转导子与激活子3 HEDGEHOG信号通路 

分 类 号：R28[医药卫生—中药学]