有柄石韦中二氢黄酮类和咖啡酰奎宁酸类5个成分的同时测定  

Simultaneous determination of five dihydroflavonoids and caffeoylquinic acids in Pyrrosia petiolosa(Christ)Ching folium

作　　者：周雷罡 欧阳兰 熊晓丽 周国平 袁铭铭 胡寿荣 ZHOU Lei-gang;OUYANG Lan;XIONG Xiao-li;ZHOU Guo-ping;YUAN Ming-ming;HU Shou-rong(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang 330029,China;Nanchang University,Nanchang 330006,China)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,南昌330029 [2]南昌大学,南昌330006

出　　处：《药物分析杂志》2021年第7期1163-1168,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：江西省重点研发计划项目(20171BBG70104)。

摘　　要：目的:建立同时测定有柄石韦中1个二氢黄酮类成分(圣草酚-7-O-β-D-吡喃葡萄糖醛酸苷)及4个咖啡酰奎宁酸类成分(绿原酸、4,5-二咖啡酰奎宁酸甲酯、3,4-二咖啡酰奎宁酸、1,2-二咖啡酰-环戊基-3-醇)含量的高效液相色谱法。方法:采用SHIMADZU C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.2%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长为330 nm,柱温30℃。结果:绿原酸、圣草酚-7-O-β-D-吡喃葡萄糖醛酸苷、4,5-二咖啡酰奎宁酸甲酯、3,4-二咖啡酰奎宁酸、1,2-二咖啡酰-环戊基-3-醇进样量分别在0.1066~2.6655μg(r=1.0000)、0.1602~4.0060μg(r=1.0000)、0.0065~0.1625μg(r=1.0000)、0.0069~0.1715μg(r=1.0000)和0.0096~0.2390μg(r=1.0000)范围内与峰面积呈现良好的线性关系;精密度(RSD≤0.7%)、稳定性(RSD≤0.4%)良好;平均回收率(n=6)分别为101.5%、100.4%、99.7%、97.3%和101.2%,RSD分别为0.21%、0.52%、0.57%、0.92%和1.0%;4批样品中绿原酸、圣草酚-7-O-β-D-吡喃葡萄糖醛酸苷、4,5-二咖啡酰奎宁酸甲酯、3,4-二咖啡酰奎宁酸和1,2-二咖啡酰-环戊基-3-醇含量范围分别为2.186~7.405、7.694~10.790、0.352~0.511、0.332~0.512和0.437~0.763 mg·g^(-1)。结论:该方法简便易行,重复性好,可作为有柄石韦药材的质量控制指标。Objective:To develop an HPLC method for simultaneous determination of one dihydroflavonoid[(±)-eriodictyol-7-O-β-D-glucuronide]and four caffeoylquinic acids(chlorogenic acid,4,5-dicaffeoylquinic acid methyl ester,3,4-di-O-caffeoylquinic acid,1,2-O-dicaffeoyl-cychopenta-3-ol)in the leaves of Pyrrosiapetiolosa(Christ)Ching.Methods:The SHIMADZU C_(18) column(4.6 mm×250 mm,5μm)was used,the mobile phase was acetonitrile(A)-0.2%phosphate aqueous(B)with gradient elution,at the flow rate of 1.0 mL·min^(-1),the detection wavelength was 210 nm,and the column temperature was at 30℃.Results:The linear ranges of chlorogenic acid and(±)-eriodictyol-7-O-β-D-glucuronide,4,5-dicaffeoylquinic acid methyl ester,3,4-di-O-caffeoylquinic acid and 1,2-O-dicaffeoyl-cychopenta-3-ol were 0.1066-2.6655μg(r=1.0000),0.1602-4.0060μg(r=1.0000),0.0065-0.1625μg(r=1.0000),0.0069-0.1715μg(r=1.0000)and 0.0096-0.2390μg(r=1.0000),respectively.The precision(RSD≤0.7%)and stability(RSD≤0.4%)were good.The average recoveries(n=6)were 101.5%(RSD=0.21%),100.4%(RSD=0.52%),99.7%(RSD=0.57%),97.3%(RSD=0.92%)and 101.2%(RSD=1.0%),respectively.The contents of chlorogenic acid,(±)-eriodictyol-7-O-β-D-glucuronide,4,5-dicaffeoylquinic acid methyl ester;3,4-di-O-caffeoylquinic acid and 1,2-O-dicaffeoyl-cychopenta-3-ol in four batches of samples were 2.186-7.405,7.694-10.790,0.352-0.511,0.332-0.512 and 0.437-0.763 mg·g^(-1),respectively.Conclusion:This method is specific,accurate and reproducible,and can be used to control the quality of the leaves of P.petiolosa.

关 键 词：有柄石韦 绿原酸 圣草酚-7-O-β-D-吡喃葡萄糖醛酸苷 4 5-二咖啡酰奎宁酸甲酯 3 4-二咖啡酰奎宁酸 1 2-二咖啡酰-环戊基-3-醇 二氢黄酮类成分 咖啡酰奎宁酸类成分 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]