半夏泻心汤对右旋葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠肠道菌群的影响  

Effect of Banxia Xiexin Decoction on intestinal flora of mice with ulcerative colitis induced by dextran sodium sulfate

作　　者：陈健[1,2] 张梁坤[1] 谷文超 张新生 李灵 韩涛 刘西建[1] 黄海量[1] CHEN Jian;ZHANG Liang-kun;GU Wen-chao;ZHANG Xin-sheng;LI Ling;HAN Tao;LIU Xi-jian;HUANG Hai-liang(Shandong University of Traditional Chinese Medicine,Ji'nan 250300,China;Department of Traditional Chinese Medicine,Ji'nan Central Hospital,Ji'nan 250013,China;Zhang Xin-sheng Chinese Medicine Clinic(Xinsheng Hall),Linqu 262600,China)

机构地区：[1]山东中医药大学,山东济南250300 [2]济南市中心医院中医科,山东济南250013 [3]临朐张新生中医诊所(新生堂),山东临朐262600

出　　处：《中国中药杂志》2021年第11期2871-2880,共10页China Journal of Chinese Materia Medica

基　　金：山东中医药大学首批科研创新优秀团队项目(220316);山东省重点研发计划项目(2016CYJS08A01-6);2020年度山东省中医药科技面上项目(2020M096);2020年齐鲁卫生与健康领军人才培育工程项目(鲁卫人才字[2020]3号);山东省高等学校科研发展计划项目(J18KB130);全国名老中医药专家刘持年传承工作室建设项目(国中医药人教发[2014]20号);山东省名老中医药专家刘昭纯传承工作室建设项目(鲁卫中发展字[2018]1号)。

摘　　要：观察半夏泻心汤对右旋葡聚糖硫酸钠诱导建立的溃疡性结肠炎(DSS-UC)小鼠模型炎症因子及肠道菌群的影响,从菌群紊乱角度探讨其治疗溃疡性结肠炎的作用机制。将40只C57BL/6J小鼠随机分为空白组10只、模型组15只和半夏泻心汤组15只,采用2.5%DSS诱导溃疡性结肠炎模型,第8天开始每日对应给予生理盐水、生理盐水、半夏泻心汤14 d后,HE染色法观察结肠组织病理学变化,ELISA法检测血清炎症因子含量,运用16S rRNA测序技术检测小鼠粪便肠道菌群的变化。与空白组比较,模型组小鼠结肠组织损伤严重,血清白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)含量显著升高(P<0.05);与模型组比较,半夏泻心汤组小鼠结肠损伤减轻,血清IL-6和TNF-α含量显著下降(P<0.05);造模后,肠道微生物在门水平上Patescibacteria丰富度显著增加(P<0.05);在属水平上Faecalibaculum(P<0.01)、norankfMuribaculaceae(P<0.01)丰富度显著减少,Turicibacter(P<0.01)、Romboutsia(P<0.01)、Clostridiumsensustricto1(P<0.01)丰富度则显著增加。半夏泻心汤干预后,在门水平上Patescibacteria明显减少(P<0.05);在属水平上,Lactobacillus(P<0.01)、Clostridiumsensustricto1(P<0.01)、Enterorhabdus(P<0.01)、CandidatusSaccharimonas(P<0.05)、Eubacteriumfissicatenagroup(P<0.05)显著减少,而Dubosiella、Bacteroides、Allobaculum则显著增加。综上所述,半夏泻心汤可以显著改善DSS-UC小鼠症状,降低IL-6和TNF-α含量;并可在门水平上降低Patescibacteria丰富度,属水平上降低Clostridiumsensustricto1、CandidatusSaccharimonas、Eubacteriumfissicatenagroup丰富度,提高Bacteroides、Bifidobacterium丰富度,进而起到减轻肠道炎症和治疗溃疡性结肠炎的作用。The aim of this paper was to investigate the effect of Banxia Xiexin Decoction(BXD) on inflammatory factors and intestinal flora in a dextran sulfate sodium induced ulcerative colitis(DSS-UC) mouse model, and to explore the mechanism of BXD in treating ulcerative colitis from the perspective of flora disorder. Forty C57 BL/6 J mice were randomly divided into control group, model group and BXD group. A 2.5% DSS-induced ulcerative colitis model was established. On the 8 th day, normal saline, normal saline, and BXD were given daily for 14 days. After 14 days, HE staining was used to observe histopathological changes of the colon. Serum inflammatory factor content was detected by ELISA, and the change of intestinal flora in mice feces was detected by 16 S rRNA sequencing technology. Compared with control group, the colonic tissue of mice in model group was damaged seriously, and the contents of IL-6 and TNF-α in serum were significantly increased(P<0.05). Compared with model group, mice in BXD group had less colonic damage, and the contents of IL-6, TNF-α in serum were decreased significantly(P<0.05). After creation, the richness of Patescibacteria was increased significantly at the phylum level(P<0.05). At the same time, the richness of Faecalibaculum(P<0.01), norankfMuribaculaceae(P<0.01) were decreased significantly at the genus level, while the richness of Turicibacter(P<0.01), Romboutsia(P<0.01), Clostridiumsensustricto1(P<0.01) were increased significantly. After the intervention with BXD, the content of Patescibacteria was significantly reduced at the phylum level(P<0.05), and the contents of Lactobacillus(P<0.01), Clostri-diumsensustricto1(P<0.01), Enterorhabdus(P<0.01), CandidatusSaccharimonas(P<0.05), Eubacteriumfissicatenagroup(P<0.05) were decreased significantly at the genus level, while the contents of Dubosiella, Bacteroides and Allobaculum were increased significantly. Therefore, BXD could significantly improve the symptoms of DSS-UC mice. It not only could reduce the contents of IL-6 and TNF-α, b

关 键 词：溃疡性结肠炎 半夏泻心汤 炎症因子 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]