人工感染PRRSV JXA1株仔猪肾脏组织病理变化及病毒分布  

Histopathological Lesions and Virus Distribution in Experimentally PRRSV-infected Piglet Renal Tissues

作　　者：吴显平 杨重锦 迟盛仁 张志欢 张永红 张涛 WU Xian-ping;YANG Zhong-jin;CHI Sheng-ren;ZHANG Zhi-huan;ZHANG Yong-hong;ZHANG Tao(Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Animal Science and Technology College,Beijing University of Agriculture,Beijing ,102206,China;Beijing Neurosurgical Institute,Beijing.100070,China)

机构地区：[1]北京农学院动物科学技术学院兽医学(中医药)北京市重点实验室,北京102206 [2]北京市神经外科研究所,北京100070

出　　处：《动物医学进展》2021年第9期30-34,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31672597);北京市自然科学基金项目(6202003);北京市教委科研计划项目(KM202010020008)。

摘　　要：为了解感染猪繁殖与呼吸综合征病毒(PRRSV)仔猪肾脏组织的主要病变和病毒主要分布的细胞类型,试验取人工感染PRRSV JXA1株的仔猪肾脏组织制作石蜡切片,采用HE染色法观察其病理变化,采用免疫组织化学方法检测病毒GP5蛋白和N蛋白在肾脏组织内的分布。结果表明,感染仔猪的肾脏组织呈现广泛性充血和出血,大量的炎症细胞浸润;感染仔猪肾脏组织的免疫组织化学染色,PRRSV GP5蛋白和N蛋白呈广泛性阳性着色,阳性细胞包括肾小球毛细血管内皮细胞、肾小管周围毛细血管内皮细胞、肾小管上皮细胞和肾间质等。说明PRRSV感染仔猪病毒粒子能够侵入肾脏组织,感染肾血管内皮细胞和肾小管上皮细胞等。To understand the main histopathological lesions and virus-infected cell types in kidneys of porcine reproductive and respiratory syndrome virus(PRRSV)-infected piglets,paraffin sections were prepared from the kidneys of experimentally PRRSV JXA1-infected piglets,and the histopathological lesions were observed by HE staining and the viral distribution was detected by immunohistochemical staining for GP5 and N proteins.Results indicated that extensive congestion and bleeding and mass inflammatory cell infiltration were observed in infected piglet renal sections.Immunohistochemical staining for GP5 and N proteins indicated extensive positive staining in infected piglet renal cells,such as renal glomerular capillary endothelial cells,peritubular capillary endothelial cells,renal tubular epithelial cells and renal interstitium.The results showed that PRRSV can invade piglet renal tissues and infect renal capillary endothelial cells and renal tubular epithelial cells.

关 键 词：猪繁殖与呼吸综合征病毒 肾脏 GP5蛋白 N蛋白 血管内皮细胞 免疫组织化学 

分 类 号：S852.31[农业科学—基础兽医学] S852.659.6[农业科学—兽医学]