HEK-293细胞培养过程中支原体污染处理初探  

Treatment of Mycoplasma contamination in HEK-293 Cell Culture

作　　者：易蓉鑫 杨齐之贤 梁易晓 周祖灵 孙永科 杨玉艾 YI Rong- xin;YANG Qi- zhi- xian;LIANG Yi- xiao;ZHOU Zu- ling;SUN Yong- ke;YANG Yu- ai(College of Veterinary Medicine,Yunnan Agricultural University,Kunming,Yunnan,650000,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650000

出　　处：《动物医学进展》2021年第9期53-57,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31760743)。

摘　　要：为探索出HEK-293细胞培养中支原体污染的快速清除方法,通过大环内酯类和四环素类2种抗生素药物的单独、交替及联合使用对污染的HEK-293细胞进行处理,应用DNA荧光染色及PCR检测,比较处理前与处理后支原体污染的情况有无改善。检测结果显示,通过10 mg/mL浓度的大环内酯类和四环素类2种药物交替使用各4 d,处理2个周期后的HEK-293细胞中支原体污染已被清除。结果表明,通过10 mg/mL的大环内酯类和四环素类2种抗生素药物的交替使用,使HEK-293细胞培养过程中的支原体污染得到有效的控制,为细胞研究及疫苗工业生产奠定了基础。Inorde to explore a rapid treatment for Mycoplasma contamination in HEK-293 cell culture,the contaminated HEK-293 cells were treated with macrolide and tetracycline antibiotics alone,alternately or in combination,and detected by DNA fluorescence staining and PCR.The results showed that the effect of Mycoplasma contamination in HEK-293 cells after two cycles of treatment was the most significant through the alternating use of macrolide and tetracycline at 1%concentration for four days each.The alternating use of 1%macrolide and tetracycline antibiotics can effectively control the Mycoplasma contamination in the cell culture of HEK-293,and lay a foundation for the follow-up cell research and vaccine industry production.

关 键 词：细胞培养 支原体 PCR检测 DNA荧光染色 

分 类 号：S852.62[农业科学—基础兽医学] S859.796[农业科学—兽医学]