温度对红耳滑龟白细胞数量和杀菌能力的影响  

Effect of Temperature on Number of Leukocytes and Bactericidal Capacity in Red-eared Slider Turtle,Trachemys scripta elegans

作　　者：吴海燕 冯文红 吴文欣 许洋溢 杨雨晴 刁迎珠 张志强[1] WU Hai-yan;FENG Wen-hong;WU Wen-xin;XU Yang-yi;YANG Yu-qing;DIAO Ying-zhu;ZHANG Zhi-qiang(College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui,230036,China)

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《动物医学进展》2021年第9期91-95,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31170379);安徽省舒城县“三区”科技人才项目(2020);安徽农业大学2020年度省级重点教学研究项目(2020jyxm0531);安徽农业大学2019年省级大学生创新创业训练计划项目创新训练项目(201910364041)。

摘　　要：为探究温度对红耳滑龟(Trachemys scripta elegans)健康状态的影响,通过活体背甲下静脉窦采血,测定了20、24、28、32℃条件下处理6周后,其身体大小、各型白细胞的百分比和血浆杀菌能力的变化。不同温度组的体重、背甲长和背甲宽均无显著的组间差异(P>0.05)。淋巴细胞的百分比24℃组显著高于20℃组(P<0.05),但嗜碱性粒细胞的百分比24℃组显著低于28℃组和20℃组(P<0.05),中性粒细胞、嗜酸性粒细胞和单核细胞的百分比,以及中性粒细胞与淋巴细胞的比值和血浆杀菌能力均无显著的组间差异(P>0.05)。红耳滑龟依赖血液中淋巴细胞和嗜碱性粒细胞数量增减来适应温度升降,这可能是其易于扩散的生理学基础之一。To explore the effects of ambient temperature on the healthy status of the red-eared slider turtles,Trachemys scripta elegans,we measured body size,percentage of different subtypes of leukocytes,and plasma bactericidal capacity with blood samples by using of the dorsal cervical sinus blood collection after 6 week temperature acclimation of 20℃,24℃,28℃or 32℃,respectively.There were no significant differences among temperature groups in body mass,carapace length and width(P>0.05).After 6-week acclimation,percentage of lymphocytes in the 24℃group was significantly greater than that of the 20℃group(P<0.05),but percentage of basophils in the 24℃group was significantly lower than that of the 28℃and 20℃groups(P<0.05),and no significant group differences were found in percentage of neutrophils,eosinophils and monocytes,as well as the ratio of neutrophils to lymphocytes and plasma bactericidal capacity(P>0.05).Red-eared slider turtles adapt to increasing or declining temperatures by the numerous increasement or reduction of lymphocytes and basophils,which may be considered as one of their physiological bases affecting their invasion diffusion in the field.

关 键 词：红耳滑龟 温度 淋巴细胞 嗜碱性粒细胞 血浆杀菌能力 

分 类 号：S852.2[农业科学—基础兽医学]