二烯丙基二硫化物对MDCC-MSB-1细胞凋亡的影响  

Effects of diallyl disulfide on apoptosisof MDCC-MSB-1 cells

作　　者：张佩琪 周鹏飞 叶正钦 刘进辉 王宜平 纪春晓 ZHANG Peiqi;ZHOU Pengfei;YE Zhengqin;LIU Jinhui;WANG Yiping;JI Chunxiao(Hunan Engineering Technology Research Center of Veterinary Drugs,College of Veterinary Medicine,Hunan Agricultural University,Changsha 410000,China)

机构地区：[1]湖南农业大学动物医学院湖南省兽药工程技术研究中心,湖南长沙410000

出　　处：《中国兽医学报》2021年第7期1354-1358,1406,共6页Chinese Journal of Veterinary Science

基　　金：湖南省自然科学基金资助项目(2020JJ4350);湖南省教育厅优秀青年资助项目(19B251);国家自然科学基金资助项目(31402258)。

摘　　要：以50,100,150μmol/L二烯丙基二硫化物(diallyl disulfide, DADS)处理MDCC-MSB-1细胞24 h后,利用吖啶橙(acridine orange, AO)染色法对细胞凋亡的形态进行初步观察;Annexin V-FITC/PI双染法检测细胞凋亡率;JC-1染色法检测线粒体膜电位的变化;Western blot检测P53的蛋白表达;蛋白活性检测试剂盒检测Caspase-3和Caspase-9的活性变化;Q-PCR检测Caspase-3和Caspase-9 mRNA的表达情况。结果显示,与空白组相比,DADS诱导MDCC-MSB-1细胞发生凋亡形态变化,增加细胞凋亡率,同时诱导p53蛋白表达量和线粒体膜电位下降,上调Caspase-3和Caspase-9基因表达水平和蛋白活性。MDCC-MSB-1 cells were treated with 50,100,150 μmol/L diallyl disulfide(DADS) for 24 h, and the apoptotic morphology of cells was observed by staining with acridine orange(AO);cell apoptosis rates were detected by Annexin V-FITC/PI double staining;changes in mitochondrial membrane potential were detected by JC-1 staining;the expression of p53 protein was detected by Western blot;the protein activity of Caspase-9 and Caspase-3 was measured by protein activity assay kit;the mRNA expression levels of Caspase-9 and Caspase-3 were analyzed by q-PCR.The results showed that compared with the blank group, after treatment with DADS,the cytoplasm of MDCC-MSB-1 cells changed from homogenization to fragmentation, and cell apoptotic rate up-regulated at the same time, the p53 expression and mitochondrial membrane potential decreased, the gene expression and protein activity of Caspase-3 and Caspase-9 increased.This study laid a theoretical foundation for further study on the use of DADS to combat Marek’s disease.

关 键 词：二烯丙基二硫化物 鸡马立克病 MDCC-MSB-1细胞 线粒体凋亡途径 

分 类 号：S852.3[农业科学—基础兽医学]