盐碱胁迫下野生大豆CBL-CIPK通路表达分析  

Analysis of CBL-CIPK Pathway Proteins in Wild Soybean With Saline-Alkali Stress

作　　者：魏志园 王宇[2] 司增志 魏莱 温晓蕾 乔亚科 李桂兰 张锴 WEI Zhiyuan;WANG Yu;SI Zengzhi;WEI Lai;WEN Xiaolei;QIAO Yake;LI Guilan;ZHANG Kai(College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066000;College of horticulture science and technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066000)

机构地区：[1]河北科技师范学院农学与生物科技学院,河北秦皇岛066000 [2]河北科技师范学院园艺科技学院,河北秦皇岛066000

出　　处：《核农学报》2021年第8期1783-1793,共11页Journal of Nuclear Agricultural Sciences

基　　金：河北省重点研发计划项目(19226365D);河北省青年拔尖人才项目(BJ2019051、BJ2018017);河北省大豆产业技术体系(326-0702-JSNTKSF)。

摘　　要：野生大豆(Glycine soja)是栽培大豆(Glycine max)的近缘野生种,含有栽培大豆中不存在的优异基因。为了明确类钙调磷酸酶B蛋白(CBL)与其互作蛋白激酶(CIPKS)组成的CBL-CIPK通路蛋白在野生大豆抗盐碱胁迫中的功能,本研究在盐碱胁迫下高耐及敏感野生大豆材料转录组分析的基础上,利用在线生物学工具对野生大豆CBL-CIPK通路蛋白结构、亚细胞定位、磷酸化位点及系统发育进行了分析。结果发现,10个野生大豆CBLs蛋白均含有3个EF手结构;7个CBLs蛋白定位于细胞核,另外3个分别定位于细胞质、叶绿体和内膜系统。38个CIPKs蛋白均含有ATP结合结构域、丝氨酸/苏氨酸蛋白激酶活性结构域和NAF结构域;20个CIPKs蛋白定位在细胞质中,9个定位在叶绿体中,6个定位在细胞核中,另有3个分别位于细胞器膜、线粒体和内膜系统。野生大豆与栽培大豆的CBL-CIPK通路基因高度同源,但野生大豆GsCIPK5like基因与栽培大豆GmCIPK5亲缘关系较远,栽培大豆GmCIPK21基因与野生大豆GsCIPK21亲缘关系较远。在盐碱胁迫处理0和10 h后,GsCBL10基因在高耐材料中的表达量比敏感材料显著上调;在盐碱胁迫处理0 h后,GsCIPK10基因在高耐材料中的表达量也比敏感材料显著上调。本研究为阐明CBL-CIPK通路对野生大豆耐盐碱胁迫中的功能奠定了理论基础。Wild soybean(Glycine soja) is the wild species of cultivated soybean(Glycine max), which contains excellent genes that do not exist in G. max. In order to clarify the functions of genes involved in CBL-CIPK pathway in tolerance to saline-alkali stress in wild soybean, the structural domains, the prediced subcellular localization, the phosphorylation sites, and the phylogenetic correlation of CBL-CIPK proteins were analyzed based on the transcriptome data of G. soja under saline-alkali stress. The results showed that ten CBLs proteins contained three EF-hands motifs, among which seven CBLs proteins were located in the nucleus, and the other three were located in cytoplasm, chloroplast and endomembrane systems respectively. 38 CIPKs proteins contained ATP binding domain, serine/threonine protein kinase active domain and NAF domain, among which 20 CIPK proteins were located in cytoplasm, nine proteins were located in chloroplast, six proteins were located in nucleus, and three proteins were located in organelle membrane, mitochondrion and endomembrane system respectively. The CBL-CIPK pathway genes of G. soja and G. max were highly homologous, but the GsCIPK5-like gene had far genetic relationship with GmCIPK5, and the GmCIPK21 gene had far genetic relationship with GsCIPK21. At 10 h after saline-alkali treatment, the expression of GsCBL10 in high-tolerance materials was significantly up-regulated than that in sensitive materials. At 0 h, the expression of GsCIPK10 in high-tolerance materials was also higher than that in sensitive materials. This study provides a theoretical foundation for clarifying the function of CBL-CIPK pathway in tolerance to saline-alkali stress G. soja.

关 键 词：盐碱胁迫 野生大豆 CBLs CIPKs 

分 类 号：S565.1[农业科学—作物学]