曲札茋苷对二氯化钴诱导PC12细胞氧化应激的保护作用  

The protective effect of Piceatannol3'-O-glucoside on PC12cells against oxidative stress induced by cobalt chloride

作　　者：翁雨天 方娟 赵水仙 黄茜 杨旭娟 付传美 刘一丹 刘军锋 Weng Yutian;Fang Juan;Zhao Shuixian(Kunming Medical University,Kunming Yunnan 650500)

机构地区：[1]昆明医科大学,650500 [2]昆药集团股份有限公司

出　　处：《卒中与神经疾病》2021年第4期378-382,共5页Stroke and Nervous Diseases

基　　金：国家重点研发计划(2017YFC1702504)。

摘　　要：目的探讨曲札茋苷注射液对二氯化钴诱导PC12细胞氧化应激的保护作用及其机制。方法取对数生长期PC12细胞随机分为5组:对照组、模型组、曲札茋苷低水平组、曲札茋苷高水平组、依达拉奉组;甲基三氯硅烷(Methyltrichlorosilane,MTS)法检测细胞活力:分光光度法检测超氧化物歧化酶(Superoxide dismutase,SOD)活性、乳酸脱氢酶(Lactate dehydrogenase,LDH)水平;荧光探针法检测活性氧(Reactive oxygen species,ROS)荧光强度;Western Blot法检测细胞中半胱氨酸天冬氨酸蛋白酶-3(Cysteinyl aspartate specific proteinase-3,Caspase-3)、B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)、Bcl-2-相关X(Bcl-2-Associated X,Bax)蛋白的表达水平。结果与对照组比较,模型组细胞活力下降,SOD活性降低,LDH释放增加,活性氧水平增高,Caspase-3,Bax蛋白的表达水平上升,Bcl-2蛋白表达水平下降(P<0.05);与模型组比较,曲札茋苷组、依达拉奉组细胞活力上升,SOD活性升高,LDH释放较少,活性氧水平降低,Caspase-3,Bax蛋白的表达水平下降,Bcl-2蛋白表达水平上升(P<0.05)。结论曲札茋苷可明显升高氧糖剥夺/复糖复氧PC12细胞的存活率,其机制可能是通过调控相关凋亡蛋白的表达,从而抑制细胞的凋亡。Objective To investigate the effect of Piceatannol3'-O-glucoside on PC12 cells against oxidative stress induced by cobalt chloride,and to explore the potential mechanisms.Methods PC12 cells were randomly divided into 5 groups:control group,model group,high and low doseof Piceatannol3'-O-glucoside,and edaravone group.The Methyltrichlorosilane,MTS(MTS)method was used to detect cell viability.The contents of superoxide dismutase(SOD)and actatedehydrogenase(LDH)were determinated by spectrophotometry.Fluorescence probe assay was applied to detect the fluorescence intensity of reactive oxygen species(ROS).Western blotting was used to detect the protein expression of Caspase-3、Bax and Bcl-2 in cells.Results Compared with the normal group,the LDH level,the ROS level,and the expression of Bax and caspase-3 were significantly increased in the model group,while the cell viability,SOD activity,andBcl-2 expression were decreased in the model group(P<0.05).In addition,compared with the model group,the LDH level,the ROS leveland the expression of Bax and caspase-3 were significantly decreased in the Piceatannol3'-O-glucoside group and edaravone group,while cell viability,SOD activity,and the expression of Bcl-2 were obviously increased in the Piceatannol3'-O-glucoside group and edaravone group(P<0.05).Conclusion Piceatannol3’-O-glucosidesignificantly improve the survival rate of oxygen-glucose deprivation/reoxygenation in PC12 cells,and the mechanism may be related to regulation of cell apoptosis-related proteins expression.

关 键 词：曲札茋苷 氧糖剥夺/复糖复氧 二氯化钴 PC12细胞 凋亡 氧化损伤 

分 类 号：R285[医药卫生—中药学] R743[医药卫生—中医学]