Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation  

作　　者：JIA Rong CHEN Ya Xiong DU Ya Rong HU Bu Rong 

机构地区：[1]Key Laboratory of Space Radiobiology of Gansu Province&CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine,Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,Gansu,China [2]School of Nuclear Science and Technology,University of Chinese Academy of Sciences,Beijing 100039,China [3]Department of Radiation Medicine,School of Public Health and Management,Wenzhou Medical University,Wenzhou 325035,Zhejiang,China

出　　处：《Biomedical and Environmental Sciences》2021年第7期540-551,共12页生物医学与环境科学（英文版）

基　　金：supported by the National Natural Science Foundation of China[11635013,11705248,U1832101];National Key Research and Development Program of China[2017YFC0108605];the Science and Technology Research Project of Gansu Province[No.145RTSA012 and 17JR5RA307]。

摘　　要：Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery(MSD),a high-throughput and sensitive electrochemiluminescence measurement technique.Dose estimation equations were set up using multiple linear regression analysis.Results The relative levels of IL-6 at 1 h,IL-5 and IL-6 at 24 h,and IL-5,IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently.The minimum measured levels of IL-5,IL-6 and IL-15 were up to 4.0076 pg/mL,16.4538 pg/mL and 0.4150 pg/mL,respectively.In addition,dose estimation models were established and verified.Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5,IL-6 and IL-15.These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators.Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.

关 键 词：MSD assay Low or high LET irradiation Mouse plasma Cytokines Dose estimation model 

分 类 号：R818[医药卫生—放射医学]