重组别藻蓝蛋白的超快能量传递过程  

Ultra-fast energy transfer process of recombinant allophycocyanin

作　　者：甄张赫 朱锐丹 秦松[1,4] 陈海龙[3] 蒲洋[5] 翁羽翔 李文军 Zhen Zhanghe;Zhu Ruidan;Qin Song;Chen Hailong;Pu Yang;Weng Yuxiang;Li Wenjun(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China;Beijing National Laboratory for Condensed Matter Physics/Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190,China;Center for Ocean Mega-Science,Chinese Academy of Sciences,Qing-dao 266071,China;School of Agriculture,Ludong University,Yantai 264025,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049 [3]中国科学院物理研究所北京凝聚态物理国家研究中心/软物质物理重点实验室,北京100190 [4]中国科学院海洋大科学研究中心,山东青岛266071 [5]鲁东大学农学院,山东烟台264025

出　　处：《海洋学报》2021年第9期115-125,共11页

基　　金：国家自然科学基金(41906109);中国科学院前沿科学重点研究项目(QYZDJ-SSW-SYS017)。

摘　　要：藻胆体是红、蓝藻特有的光合作用捕光天线复合物。别藻蓝蛋白(APC)是组成藻胆体高效能量传递的核心结构的主要成分。本文以基因重组的别藻蓝蛋白(rAPC)的单体和三聚体为材料,通过稳态光谱、圆二色光谱以及超快时间分辨光谱研究了rAPC的结构构象和能量传递过程。结果表明,rAPC在测试条件下能保持和天然APC一致的光谱特性和活性构象;rAPC单体组装成三聚体后,其α^(84)PCB和β^(84)PCB可以组成激子色素对,通过激子分裂提高三聚体的能量传递效率;超快时间分辨光谱结果显示,在rAPC三聚体中,能量从620 nm传至650 nm的时间为300~600 fs,同时存在着19 fs的激子态的电子退相干过程。这些结果为揭示藻胆体的高效能量传递机制提供了数据基础。Phycobilisomes are photosynthetic light-harvesting antenna complexes unique to red algae and cyanobacteria.Allophycocyanin(APC)is the main component of the core structure of phycobilisomes.In this experiment,recombinant allophycocyanin(rAPC)was used as the material,and the structure conformation and energy transfer process of rAPC were studied through steady-state spectroscopy,circular dichroic spectroscopy,and ultrafast time-resolved spectroscopy.The results show that rAPC can maintain the same spectral characteristics and active conformation as natural APC under the test conditions;after rAPC monomers are assembled into trimer,the results confirmed thatα^(84)PCB andβ^(84)PCB can form an exciton pigment pair which can improve the energy transfer efficiency of the rAPC trimer through exciton splitting;ultrafast time-resolved spectroscopy results show that the energy transfer time from 620 nm to 650 nm is 300−600 fs,and there is also a 19 fs exciton state electronic decoherence process.These results provide a data basis for revealing the efficient energy transfer mechanism of phycobilisomes.

关 键 词：别藻蓝蛋白 重组异源表达 能量传递 稳态光谱 瞬态光谱 

分 类 号：Q949.2[生物学—植物学] Q946.1