基于网络药理学的双黄杞贞克瘤方抗肿瘤机制  

The anti-tumour mechanism of ShuanghuangQizhen Keliu Prescription based on network pharmacology

作　　者：郎晓娜 邱玉玲[1] 孔德新[1] LANG Xiao-na;QIU Yu-ling;KONG De-xin(School of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Dept of Pharmacy,Tianjin Hospital,Tianjin 300211,China)

机构地区：[1]天津医科大学药学院,天津300070 [2]天津市天津医院药学部,天津300211

出　　处：《中国药理学通报》2021年第9期1311-1317,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 82073890,81673464,81973570)。

摘　　要：目的基于网络药理学方法探讨双黄杞贞克瘤方的抗肿瘤作用机制。方法利用TCMSP数据库筛选出双黄杞贞克瘤方中5种药物的主要活性成分及作用靶点,利用OMIM等3个数据库检索肿瘤相关基因,绘制韦恩图对药物靶点与疾病靶点进行匹配,找出潜在的核心靶点。采用Cytoscape软件构建网络图并绘制找出核心靶点。在Metascape平台对核心靶点进行GO和KEGG富集分析,通过OmicShare平台对结果进行通路分析。 结果 组方中筛选出槲皮素、山奈酚、β-谷甾醇等主要活性成分作用于TP53、AKT1、JUN、MAPK1、TNF、RELA、IL6等靶点,通过抑制肿瘤细胞增殖、迁移,诱导凋亡发挥抗肿瘤作用。 结论 初步探讨了双黄杞贞克瘤方抗肿瘤机制,为实验研究提供了思路和依据。Aim To explore the anti-tumour mechanism of Shuanghuang Qizhen Keliu Prescription based on network pharmacology.Methods The active components and targets of Shuanghuang Qizhen Keliu Prescription were searched and selected through the TCMSP database.Related targets of tumor were screened and retrieved by OMIM.The key anti-tumor targets of Shuanghuang Qizhen Keliu Prescription were obtained by using Venn diagram analysis.The key target PPI network was constructed and the key targets were obtained by Cytoscape.Metascape platform was used for GO and KEGG enrichment analysis of key targets.Results There were many active components such as quercetin,kaempferol,and beta-sitosterol in Shuanghuang Qizhen Keliu Prescription,and their targets might be TP53,AKT1,JUN,MAPK1,TNF,RELA,IL-6 and so on.The anti-tumour mechanism might be proliferation inhibition,migration inhibition and apoptosis promotion.Conclusions This study preliminarily discusses the potential anti-tumour mechanism of Shuanghuang Qizhen Keliu Prescription,providing ideas and basis for subsequent experimental research.

关 键 词：网络药理学 黄芪 黄精 枸杞子 女贞子 灵芝 肿瘤 

分 类 号：R285.5[医药卫生—中药学] R289.1[医药卫生—中医学]