慢性非细菌性前列腺炎大鼠前列腺线粒体损伤及解毒活血汤干预作用研究  

Jiedu Huoxue Decoction improves mitochondrial damage of the prostate in rats with chronic nonbacterial prostatitis

作　　者：董德刚[1] 宋梅[2] 易军[3] 严张仁[3] 王万春[3] DONG De-gang;SONG Mei;YI Jun;YAN Zhang-ren;WANG Wan-chun(Faculty of Biochemistry,School of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nan-chang,Jiangxi 330004,China;Department of Humanities,School of Science arid Technology,Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330004,China;Department of Andrology,The Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330006,China)

机构地区：[1]江西中医药大学中医学院生物化学教研室,江西南昌330004 [2]江西中医药大学科技学院人文系,江西南昌330004 [3]江西中医药大学附属医院男性病科,江西南昌330006

出　　处：《中华男科学杂志》2021年第6期483-488,共6页National Journal of Andrology

基　　金：国家自然科学基金(81960873)。

摘　　要：目的:从线粒体角度探讨慢性非细菌性前列腺炎(CNP)大鼠可能发病机制,以及解毒活血汤干预作用。方法:清洁级SD雄性大鼠随机分为4组,即假手术组、模型组、阳性药组及解毒活血汤组,采用前列腺内注射角叉菜胶法制备CNP大鼠模型,其中假手术组和模型组灌服生理盐水,阳性药组则按2 g/kg灌服前列康片,解毒活血汤组按生药量2 g/kg灌服解毒活血汤,每天1次,连续给药30 d后处死,收集血清与前列腺组织,计算各组前列腺组织脏器系数比,并分别进行常规HE染色,采用线粒体提取试剂盒提取各组大鼠前列腺组织线粒体,比色法检测前列腺线粒体超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)水平及Na^(+)-K^(+)-ATPase活性。透射电镜观察前列腺超微结构变化。结果:前列腺脏器系数假手术组[(1.50±0.42)%]、阳性药组[(1.54±0.32)%]和解毒活血汤组[(1.47±0.53)%]均显著低于模型组[(1.95±0.39)%,P<0.05];光镜下显示假手术组大鼠前列腺组织无明显病理变化,模型组间质内纤维组织明显增生,腺体结构紊乱,炎性细胞浸,两药物治疗组炎性细胞减少,腺泡内上皮细胞与间质内纤维组织增生程度改善;模型组大鼠前列腺组织线粒体SOD、GSH-PX、Na^(+)-K^(+)-ATPase活性均低于其他组(P<0.05或P<0.01),其中解毒活血汤组与模型组比较[SOD:(22.52±3.88)U/mg prot vs(17.42±2.91)U/mg prot,P<0.05;GSH-Px:(89.95±7.65)U/mg prot vs(38.35±6.98)U/mg prot,P<0.01;Na^(+)-K^(+)-ATPase:(1.85±0.32)μmol Pi/mg prot/hvs(0.98±0.40)μmol Pi/mg prot/h,P<0.01],MDA含量均高于其他各组(P<0.01),其中解毒活血汤组与模型组MDA比较为(0.59±0.17)nmol/mg prot vs(1.70±0.22)nmol/mg prot;超微结构显示,模型组大鼠前列腺组织有显著线粒体损伤,解毒活血汤能诱导线粒体自噬增强。结论:CNP大鼠前列腺存在线粒体功能障碍,解毒活血汤能促进CNP大鼠前列腺线粒体功能恢复,其机制可能与线Objective:To explore the possible pathogenesis of chronic nonbacterial prostatitis(CNP)in rats from the perspective of mitochondria,and the interventional effect of Jiedu Huoxue Decoction(JHD)on CNP.Methods:Forty clean-grade SD male rats were randomly divided into 4 groups of an equal number,sham control,CNP model control,Qianliekang Tablets intervention(QLK)and JHD intervention,those in the former two groups treated intragastrically with normal saline,and those in the latter two with QLK and JHD,respectively,at 2 g/kg qd for 30 successive days.Then serum and prostate tissue samples were collected from the rats for calculation of the organ coefficients,HE staining,extraction of mitochondria in the prostate tissue,measurement of the levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-PX)and Na^(+)-K^(+)-ATPase by colorimetric assay,and observation of the ultrastructural changes of the prostatic epithelial cells under the transmission electron microscope(TEM).Results:The organ coefficient of the prostate was significantly higher in the CNP model controls([1.95±0.39]%)than in the sham control([1.50±0.42]%,P<0.05),QLK([1.54±0.32]%,P<0.05)and JHD groups([1.47±0.53]%,P<0.05).TEM showed significant hyperplasia of the interstitial fibrous tissue,glandular structural disorder and inflammatory cell immersion in the CNP model controls,decreased inflammatory cells and reduced hyperplasia of epithelial cells in the acinar and interstitial fibrous tissues in the QLK and JHD groups,but no significant changes in the sham controls.The CNP model controls,compared with the QLK and JHD groups,exhibited remarkably lower levels of SOD([17.42±2.91]vs[23.47±5.79]and[22.52±3.88]U/mg prot,P<0.05),GSH-PX([38.35±6.98]vs[47.68±10.37]and[89.95±7.65]U/mg prot,P<0.05 or P<0.01),and Na^(+)-K^(+)-ATPase in the prostatic mitochondria([0.98±0.40]vs[1.37±0.29]and[1.85±0.32]μmol Pi/mg prot/h,P<0.05 or P<0.01),but a higher level of MDA([1.70±0.22]vs[0.54±0.14]and[0.59±0.17]nmol/mg prot,P<0.01).Significa

关 键 词：慢性非细菌性前列腺炎 线粒体损伤 氧化应激 解毒活血汤 线粒体自噬 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]