水稻响应热胁迫核心基因的筛选与鉴定  

Screening and identification of core genes responding to heat stress in rice

作　　者：张斌[1] 杨昕霞 袁志辉[1] ZHANG Bin;YANG Xin-xia;YUAN Zhi-hui(Hunan University of Science and Engineering,Yongzhou 425199,China)

机构地区：[1]湖南科技学院,湖南永州425199

出　　处：《江苏农业学报》2021年第4期817-822,共6页Jiangsu Journal of Agricultural Sciences

基　　金：湖南省自然科学基金项目(2020JJ4030);湖南省创新平台与人才计划项目(2020NK4222)。

摘　　要：基于热胁迫条件下的水稻转录组数据,通过HTSeq和DESeq软件筛选差异表达基因;对其进行功能富集和构建蛋白质互作网络,鉴定最重要模块中的核心基因。结果显示:水稻热胁迫0 h、1 h、6 h和12 h条件下,共有278个差异表达基因,生物学过程主要富集在刺激反应和蛋白质折叠上;鉴定出含有12个基因的重要模块.基因表达模式显示重要模块中7个核心基因受热胁迫诱导上调,推测这7个核心基因在水稻响应热胁迫过程中发挥关键作用。In this study,rice transcriptome data under heat stress were used to screen differentially expressed genes by HTSeq and DESeq software,then functional enrichment analysis was carried out and protein interaction network was constructed to identify the core genes in most important module.The results showed that there were 278 differentially expressed genes under heat stress for 0 h,1 h,6 h and 12 h,and the biological process was mainly concentrated in stimulation response and protein folding.The important module containing 12 genes was identified.The gene expression patterns showed that seven core genes in important modules were up-regulated under heat stress,suggesting that these seven core genes played a key role in the response of rice to heat stress.

关 键 词：水稻 高温胁迫 核心基因 转录组 

分 类 号：S511.01[农业科学—作物学]