应用real-time PCR定量检测果园葡萄霜霉病菌潜伏侵染  

Quantitative detection of latent infection of grape downy mildew in field by real-time PCR

作　　者：杜娟 李金 张涛[1] 张游 姚珮 顾沛雯[1] DU Juan;LI Jin;ZHANG Tao;ZHANG You;YAO Pei;GU Pei-wen(College of Agronomy,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《江苏农业学报》2021年第4期861-866,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发项目(2019YFD1002502);宁夏回族自治区科技重大专项(2019BBF02013)。

摘　　要：为明确葡萄霜霉病菌潜伏侵染阶段的菌量与病害发生的关系,本研究对贺兰山东麓宁夏立兰酒庄酿酒葡萄园3个试验样地进行采样调查,分析3个样地检测的分子病情指数(MDI)与果园田间调查的病情指数(DI)的相关性。结果表明,宁夏立兰酒庄酿酒葡萄园3个样地的MDI和DI呈极显著相关,3个样地的MDI均与采样后15 d的DI拟合性最高;当MDI值为0.0035~0.1841时,采样后12 d葡萄霜霉病在果园零星发生。MDI大于0.0035时,可作为当地酿酒葡萄霜霉病防治预警指标。In order to clarify the relationship between the latent infection amount of grape downy mildew in the orchard and the occurrence of the disease,three sample plots in Ningxia Lilan vineyard were investigated,and the correlation between molecular-detected disease index(MDI)and orchard disease index(DI)was analyzed.The results showed that there was a very significant correlation between MDI and DI in three sample plots of Ningxia Lilan vineyard,and the MDI of the three sample plots had the highest fitting with the DI on the 15th day after sampling.When the MDI value ranged from 0.0035 to 0.1841,grape downy mildew occurred sporadically in the orchard on the 12th day after sampling.When MDI is greater than 0.0035,it can be used as an early warning index for prevention and control of local wine grape downy mildew.

关 键 词：葡萄霜霉菌 real-time PCR 分子病情指数 田间病情指数 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]