橡胶树NIPs通道蛋白的生物功能分析  

Functional Analysis of NOD26-like Intrinsic Proteins in Rubber Tree

作　　者：史翔宇 龙翔宇[2] 方永军[2] 阳江华[2] 肖小虎[2] 秦云霞[2] SHI Xiangyu;LONG Xiangyu;FANG Yongjun;YANG Jianghua;XIAO Xiaohu;QIN Yunxia(College of horticulture,Hainan University,Haikou,Hainan 570228,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture&Rural Affairs/Rubber Research Institute,Chinese Academy of Tropical Agricultural Sci-ences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学园艺学院,海南海口570228 [2]农业农村部橡胶树生物学与遗传资源利用重点实验室/中国热带农业科学院橡胶研究所,海南海口571101

出　　处：《热带作物学报》2021年第8期2126-2133,共8页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金面上项目(No.31770709;No.31971680);中央级公益性科研院所基本科研业务费专项(No.1630022017001)。

摘　　要：根瘤素26-like内在蛋白(nodulin 26-like intrinsic proteins,NIPs)是水通道蛋白(aquaporin protein,AQP)中一个植物特有的亚类,主要定位在质膜或内质网膜上,负责转运不带电荷的类金属溶质如硼、甘油、尿素、硅、砷、H2O2、NH3等,在植物营养获取或胁迫应答过程中发挥着重要作用。本研究以拟南芥NIPs序列为参照,利用多种生物信息学软件,对橡胶树7个HbNIPs的基因结构、蛋白理化性质、聚类分析、组织表达及割胶处理的应答进行综合分析。根据分析结果可以初步推断:7个HbNIPs应是有功能的转运蛋白,它们不但具有AQP典型的跨膜结构域(4~6个),保守的NPA基序和关键的ar/R筛选氨基酸,而且从聚类的结果可以推断它们具有转运硼酸、尿素和乳酸的功能。另外,从其启动子调控元件、表达分析和翻译后修饰分析可知,它们的活性及表达具有受发育阶段、多种激素和磷酸化修饰的严谨调控。该初步结果有助于进一步研究橡胶树NIP家族基因的功能及其在橡胶树创新育种中的应用。Nodulin 26-like intrinsic proteins(NIPs)are one subclass of plant specific aquaporin protein(AQP),mainly located at cytoplasm or endoplasmic reticulum,in charge of exchange metalloids such as boron,silicon,arsenic,selenium,glycerol,carbamide,H2O2,NH3 etc.,which are indispensible for plant microelement nutrition and stress responses.In this study,seven NIPs homologues of Arabidopsis were screened out on the rubber tree whole genome level,then bioinformations of their gene structure,protein domains and motifs,promter cis-acting elements and expression characteristics were in-depth analyzed through on-line softwares and PRISM.Totally,the seven HbNIPs were putative functional NIPs,because they owned all or partial typical characteristics of AQPs and clustered together with the homologues,such as typical domains for transmembrane,conservative NPA motifs and key ar/R amino acid for substrate determination.From the phylogenetic analysis results,they were predicated to be possibly involved in metalloids assimi-lation such as boron or glycerol,or lactate.In addition,from the analyses of cis-acting elements in the promoters,expression profiles and post-translational modifications,it is obvious that the activities would be critically regulated by development stages or hormones or phosphorylation.The primary results would benefit for further study on the function and utilization in rubber trees innovative breeding.

关 键 词：橡胶树 根瘤素26-like内在蛋白 水通道蛋白 类金属元素 

分 类 号：S794.1[农业科学—林木遗传育种]