栀子根结线虫病的病原鉴定  

Identification of Root-knot Nematode of Gardenia jasminoides Eills

作　　者：陆秀红[1] 黎达境 黄金玲[1] 李红芳 张禹[1] 刘志明[1] LU Xiuhong;LI Dajing;HUANG Jinling;LI Hongfang;ZHANG Yu;LIU Zhiming(Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning,Guangxi 530007,China;Agricultural Bureau of Hezhou,Hezhou,Guangxi 542899,China)

机构地区：[1]广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,广西南宁530007 [2]贺州市农业农村局,广西贺州542899

出　　处：《热带作物学报》2021年第8期2356-2361,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31860492);广西农业科学院基金项目(No.2021JM14,No.2021YT062)。

摘　　要：为明确广西贺州市栀子根结线虫病病原种类,采集根部有明显根结的栀子根系进行根结线虫分离鉴定,通过观察根结线虫2龄幼虫、雌成虫、会阴花纹特征对其进行形态学鉴定,并利用核糖体ITS区和28S rDNA D2D3区序列比对和系统发育树分析方法对其进行分子生物学鉴定。结果表明,该病原线虫2龄幼虫和雌成虫形态特征及形态测量值与象耳豆根结线虫(Meloidogyne enterolobii Yang&Eisenback,1983)相似;该病原线虫核糖体ITS区与NCBI数据库中象耳豆根结线虫相应序列的相似度为100%,28S rDNA D2D3区与NCBI数据库中象耳豆根结线虫相应序列的相似度为99%以上;该病原线虫核糖体ITS序列以99%的支持率与象耳豆根结线虫聚为同一分支。综合形态学和分子生物学鉴定结果将广西贺州市栀子根结线虫病病原种类鉴定为象耳豆根结线虫,栀子(Gardenia jasminoides Eills)是该线虫的新寄主纪录。In order to clarify the pathogen species of Gardenia jasminoides Eills root-knot nematode disease in Hezhou,Guangxi Province,the root samples with obvious root-knot of G.jasminoides were collected for isolating root-knot nematodes.The pathogenic nematodes were identified by observing the morphological characteristics of females,sec-ond-stage juveniles and perineal pattern,sequence alignments,phylogenetic analysis.The results showed that the mor-phological characteristics and morphometric values of the second-stage juveniles and females were similar to those of Meloidogyne enterolobii Yang&Eisenback.The rDNA-ITS fragment and 28S rDNA D2D3 fragment of the nematode were highly similar to those M.enterolobii in NCBI database,with a similarity of 100%and over 99%,respectively.The rDNA-ITS sequences of the nematode were clustered within the same clade and M.enterolobii with support rate of 99%.Morphological and molecular identification methods identified the pathogen species of G.jasminoides root-knot nema-tode disease in Hezhou as M.enterolobii.M.enterolobii is the new host for G.jasminoides Eills.

关 键 词：栀子 象耳豆根结线虫 种类鉴定 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]